000088037 001__ 88037 000088037 005__ 20200224103750.0 000088037 037__ $$aTAZ-TFG-2019-2846 000088037 041__ $$aeng 000088037 1001_ $$aEspiau Romera, Pilar 000088037 24200 $$aThe impact of high-salt diet on Type I Diabetes 000088037 24500 $$aThe impact of high-salt diet on Type I Diabetes 000088037 260__ $$aZaragoza$$bUniversidad de Zaragoza$$c2019 000088037 506__ $$aby-nc-sa$$bCreative Commons$$c3.0$$uhttp://creativecommons.org/licenses/by-nc-sa/3.0/ 000088037 520__ $$aToday, autoimmune diseases are a serious problem for humanity and constitute severe, sometimes debilitating health complications. Approximately 6% of the population has an autoimmune disease and the incidence is increasing worldwide. One of the reasons for their development is the improper regulation of the immune system, in which regulatory T cells (Tregs) play an important role. Genetic and environment factors have a major impact on the initiation and progression of these diseases. Developed countries create a milieu in which cardiovascular, metabolic, and autoimmune diseases flourish. In particular, Western diet, including high-fat, high-sugar, high-protein, and high-salt consumption, along with regular consumption of processed and ‘fast foods’, encourages the development of obesity, metabolic syndrome, and cardiovascular morbidity and mortality. In some recent studies, high-salt diet has also been reported to influence the symptoms of autoimmune diseases and decrease Treg functionality. Therefore, the aim of the current project was to study whether a high-salt diet could trigger the development of autoimmune diseases in the non-obese diabetic (NOD) mouse model, focusing in this dissertation on the development of type 1 diabetes.<br />Here, we found that a high-salt diet did not elicit type 1 diabetes development in 16-week-old NOD mice with ongoing islet inflammation. These results were unexpected as mice fed with a similar high-salt regimen developed a more severe type of experimental autoimmune encephalomyelitis, an animal model for multiple sclerosis. Moreover, high-salt intake aggravated the symptoms of systemic lupus erythematosus, collagen-induced arthritis and experimental colitis. We assume however that the composition of the HSD and especially the origin of its proteins being 24% casein might have impeded the observations. When maintained on a standard chow, which normally contains natural non-purified ingredients, like wheat middlings, wheat germ, and soybeans, NOD mice have the greatest diabetes incidence. In contrast, the introduction of semi-purified casein- or hydrolyzed casein-based diets are the least diabetogenic when mice are maintained on these diets from a very young age. Although our mice were kept until 16 week of age on a natural ingredient diet, the switch to a diet with casein as the major protein source might have prevented the further development of type 1 diabetes.<br /><br /> 000088037 521__ $$aGraduado en Biotecnología 000088037 540__ $$aDerechos regulados por licencia Creative Commons 000088037 700__ $$aMathieu, Chantal$$edir. 000088037 700__ $$aGysemans, Conny$$edir. 000088037 7102_ $$aUniversidad de Zaragoza$$b $$c 000088037 8560_ $$f719145@celes.unizar.es 000088037 8564_ $$s1005386$$uhttps://zaguan.unizar.es/record/88037/files/TAZ-TFG-2019-2846.pdf$$yMemoria (eng) 000088037 909CO $$ooai:zaguan.unizar.es:88037$$pdriver$$ptrabajos-fin-grado 000088037 950__ $$a 000088037 951__ $$adeposita:2020-02-24 000088037 980__ $$aTAZ$$bTFG$$cCIEN 000088037 999__ $$a20190722185143.CREATION_DATE