000089832 001__ 89832
000089832 005__ 20220426091134.0
000089832 0247_ $$2doi$$a10.1111/tbed.13619
000089832 0248_ $$2sideral$$a118210
000089832 037__ $$aART-2020-118210
000089832 041__ $$aeng
000089832 100__ $$aAsín, J.
000089832 245__ $$aAn outbreak of abortions, stillbirths and malformations in a Spanish sheep flock associated with a bovine viral diarrhoea virus 2-contaminated orf vaccine
000089832 260__ $$c2020
000089832 5060_ $$aAccess copy available to the general public$$fUnrestricted
000089832 5203_ $$aBovine viral diarrhoea virus (BVDV) is a pestivirus that affects both cattle and sheep, causing an array of clinical signs, which include abortions and malformations in the offspring. Manufacturing of modified live virus (MLV) vaccines often includes the use of bovine-derived products, which implies a risk of contamination with viable BVDV. Recently, the circulation of a specific strain of BVDV 2b among Spanish sheep flocks, associated with outbreaks of abortions and malformations, and whose origin was not determined, has been observed. On February 2018, a MLV orf vaccine was applied to a 1, 600 highly prolific sheep flock in the Northeast of Spain that included 550 pregnant ewes. In May 2018, during the lambing season, an unusual high rate (72.7%) of abortions, stillbirths, congenital malformations and neurological signs in the offspring was observed. It was estimated that about 1, 000 lambs were lost. Three 1- to 3-day-old affected lambs and a sealed vial of the applied vaccine were studied. Lambs showed variable degrees of central nervous system malformations and presence of pestiviral antigen in the brain. Molecular studies demonstrated the presence of exactly the same BVDV 2b in the tissues of the three lambs and in the orf vaccine, thus pointing to a pestivirus contamination in the applied vaccine as the cause of the outbreak. Interestingly, sequencing at the 5'-untranslated region-(UTR) of the contaminating virus showed a complete match with the virus described in the previously reported outbreaks in Spain, thus indicating that the same contaminated vaccine could have also played a role in those cases. This communication provides a clear example of the effects of the application of this contaminated product in a sheep flock. The information presented here can be of interest in putative future cases of suspected circulation of this or other BVDV strains in ruminants.
000089832 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000089832 590__ $$a5.005$$b2020
000089832 591__ $$aVETERINARY SCIENCES$$b4 / 146 = 0.027$$c2020$$dQ1$$eT1
000089832 591__ $$aINFECTIOUS DISEASES$$b23 / 92 = 0.25$$c2020$$dQ1$$eT1
000089832 592__ $$a1.391$$b2020
000089832 593__ $$aVeterinary (miscellaneous)$$c2020$$dQ1
000089832 593__ $$aMedicine (miscellaneous)$$c2020$$dQ1
000089832 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/submittedVersion
000089832 700__ $$aHilbe, M.
000089832 700__ $$ade Miguel, R.
000089832 700__ $$0(orcid)0000-0002-2685-1730$$aRodríguez-Largo, A.$$uUniversidad de Zaragoza
000089832 700__ $$aLanau, A.
000089832 700__ $$aAkerman, A.
000089832 700__ $$aStalder, H.
000089832 700__ $$aSchweizer, M.
000089832 700__ $$0(orcid)0000-0002-2053-9842$$aLuján, L.$$uUniversidad de Zaragoza
000089832 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000089832 773__ $$g68, 2 (2020), 233-239$$pTransboundary and Emerging Diseases$$tTransboundary and Emerging Diseases$$x1865-1674
000089832 8564_ $$s531723$$uhttps://zaguan.unizar.es/record/89832/files/texto_completo.pdf$$yPreprint
000089832 8564_ $$s287923$$uhttps://zaguan.unizar.es/record/89832/files/texto_completo.jpg?subformat=icon$$xicon$$yPreprint
000089832 909CO $$ooai:zaguan.unizar.es:89832$$particulos$$pdriver
000089832 951__ $$a2022-04-26-08:53:11
000089832 980__ $$aARTICLE