Maternal proteomic profiling reveals alterations in lipid metabolism in late-onset fetal growth restriction

Paules, C. (Universidad de Zaragoza) ; Youssef, L. ; Miranda, J. ; Crovetto, F. ; Estanyol, J.M. ; Fernandez, G. ; Crispi, F. ; Gratacós, E.

Resumen: Fetal growth restriction defined as the failure to achieve the fetal genetic growth potential is a major cause of perinatal morbidity and mortality. The role of maternal adaptations to placental insufficiency in this disorder is still not fully understood. We aimed to investigate the biological processes and protein–protein interactions involved in late-onset fetal growth restriction in particular. We applied 2D nano LC–MS/MS proteomics analysis on maternal blood samples collected at the time of delivery from 5 singleton pregnancies with late-onset fetal growth restriction and 5 uncomplicated pregnancies. Data were analyzed using R package “limma” and Ingenuity Pathway Analysis. 25 proteins showed significant changes in their relative abundance in late-onset fetal growth restriction (p value < 0.05). Direct protein–protein interactions network demonstrated that Neurogenic locus notch homolog protein 1 (NOTCH1) was the most significant putative upstream regulator of the observed profile. Gene ontology analysis of these proteins revealed the involvement of 14 canonical pathways. The most significant biological processes were efflux of cholesterol, efflux of phospholipids, adhesion of blood cells, fatty acid metabolism and dyslipidemia. Future studies are warranted to validate the potential role of the detected altered proteins as potential therapeutic targets in the late-onset form of fetal growth restriction.

Idioma: Inglés

DOI: 10.1038/s41598-020-78207-3

Año: 2020

Publicado en: Scientific Reports 10, 1 (2020), 21033 [8 pp]
ISSN: 2045-2322

Factor impacto JCR: 4.379 (2020)

Categ. JCR: MULTIDISCIPLINARY SCIENCES rank: 17 / 73 = 0.233 (2020) - Q1 - T1

Factor impacto SCIMAGO: 1.24 - Multidisciplinary (Q1)

Financiación: info:eu-repo/grantAgreement/ES/ISCIII-FEDER/Una manera de hacer Europa
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/JR19-00006
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/PIE15-00027
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/PI14-00226
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/PI15-00130
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/PI17-00675
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/PI18-00073

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Obstetricia y Ginecología (Dpto. Cirugía)