000100709 001__ 100709
000100709 005__ 20230519145404.0
000100709 0247_ $$2doi$$a10.3389/fmicb.2020.620049
000100709 0248_ $$2sideral$$a123187
000100709 037__ $$aART-2021-123187
000100709 041__ $$aeng
000100709 100__ $$aLazaro-Anton, L.
000100709 245__ $$aGlucose Oxidation to Pyruvate Is Not Essential for Brucella suis Biovar 5 Virulence in the Mouse Model
000100709 260__ $$c2021
000100709 5060_ $$aAccess copy available to the general public$$fUnrestricted
000100709 5203_ $$aBrucella species cause brucellosis, a worldwide extended zoonosis. The brucellae are related to free-living and plant-associated alpha 2-Proteobacteria and, since they multiply within host cells, their metabolism probably reflects this adaptation. To investigate this, we used the rodent-associated Brucella suis biovar 5, which in contrast to the ruminant-associated Brucella abortus and Brucella melitensis and other B. suis biovars, is fast-growing and conserves the ancestral Entner-Doudoroff pathway (EDP) present in the plant-associated relatives. We constructed mutants in Edd (glucose-6-phosphate dehydratase; first EDP step), PpdK (pyruvate phosphate dikinase; phosphoenolpyruvate pyruvate), and Pyk (pyruvate kinase; phosphoenolpyruvate -> pyruvate). In a chemically defined medium with glucose as the only C source, the Edd mutant showed reduced growth rates and the triple Edd-PpdK-Pyk mutant did not grow. Moreover, the triple mutant was also unable to grow on ribose or xylose. Therefore, B. suis biovar 5 sugar catabolism proceeds through both the Pentose Phosphate shunt and EDP, and EDP absence and exclusive use of the shunt could explain at least in part the comparatively reduced growth rates of B. melitensis and B. abortus. The triple Edd-PpdK-Pyk mutant was not attenuated in mice. Thus, although an anabolic use is likely, this suggests that hexose/pentose catabolism to pyruvate is not essential for B. suis biovar 5 multiplication within host cells, a hypothesis consistent with the lack of classical glycolysis in all Brucella species and of EDP in B. melitensis and B. abortus. These results and those of previous works suggest that within cells, the brucellae use mostly 3 and 4 C substrates fed into anaplerotic pathways and only a limited supply of 5 and 6 C sugars, thus favoring the EDP loss observed in some species.
000100709 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A14$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2014-58795-C4-1-R$$9info:eu-repo/grantAgreement/ES/MINECO/PID2019-107601RA-C32
000100709 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000100709 590__ $$a6.064$$b2021
000100709 592__ $$a1.314$$b2021
000100709 594__ $$a8.2$$b2021
000100709 591__ $$aMICROBIOLOGY$$b34 / 138 = 0.246$$c2021$$dQ1$$eT1
000100709 593__ $$aMicrobiology (medical)$$c2021$$dQ1
000100709 593__ $$aMicrobiology$$c2021$$dQ1
000100709 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000100709 700__ $$ade Miguel, M.J.
000100709 700__ $$aBarbier, T.
000100709 700__ $$aConde-Alvarez, R.
000100709 700__ $$aMunoz, P.M.
000100709 700__ $$aLetesson, J.J.
000100709 700__ $$aIriarte, M.
000100709 700__ $$aMoriyon, I.
000100709 700__ $$aZuniga-Ripa, A.
000100709 773__ $$g11 (2021), 620049 [9 pp]$$pFront. microbiol.$$tFrontiers in Microbiology$$x1664-302X
000100709 8564_ $$s283664$$uhttps://zaguan.unizar.es/record/100709/files/texto_completo.pdf$$yVersión publicada
000100709 8564_ $$s2254454$$uhttps://zaguan.unizar.es/record/100709/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000100709 909CO $$ooai:zaguan.unizar.es:100709$$particulos$$pdriver
000100709 951__ $$a2023-05-18-13:44:15
000100709 980__ $$aARTICLE