Co-immobilization and Colocalization of Multi-Enzyme Systems for the Cell-Free Biosynthesis of Aminoalcohols

Velasco-Lozano, S. ; Santiago-Arcos, J. ; Mayoral, J.A. (Universidad de Zaragoza) ; López-Gallego, F.

Resumen: The manufacturing of aminoalcohols is an appealing transformation for industrial biocatalysis due to their high value as building blocks in synthetic chemistry. By co-immobilizing dehydrogenases, ¿-transaminases and oxidases on porous carriers, we fabricated and characterized a multi-functional heterogeneous biocatalyst that was further applied to the cell-free biosynthesis of amines and aminoalcohols from alcohols and diols, respectively. This immobilized cascade integrates both redox cofactor and amine donor recycling systems into the amino alcohol biosynthesis. Spatial co-localization of the multi-enzyme system significantly increases the cofactor recycling efficiency, the system productivity and the product conversion. Using this multi-functional heterogeneous biocatalyst, we achieved 80 % conversion of alcohols into amines and accessed a palette of up to 5 aminoalcohols starting from their corresponding diols in one-pot. The results herein presented contribute to understanding the effects of the confinement of co-immobilized enzymes on the cascade processes.

Idioma: Inglés

DOI: 10.1002/cctc.201902404

Año: 2020

Publicado en: ChemCatChem 12, 11 (2020), 3030-3041
ISSN: 1867-3880

Factor impacto JCR: 5.686 (2020)

Categ. JCR: CHEMISTRY, PHYSICAL rank: 50 / 162 = 0.309 (2020) - Q2 - T1

Factor impacto SCIMAGO: 1.497 - Catalysis (Q1) - Physical and Theoretical Chemistry (Q1) - Organic Chemistry (Q1) - Inorganic Chemistry (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA/E37-17R
Financiación: info:eu-repo/grantAgreement/ES/DGA-FEDER/Construyendo Europa desde Aragón
Financiación: info:eu-repo/grantAgreement/EC/Era-CoBiotech/HOMBIOCAT/PCI2018-092984
Financiación: info:eu-repo/grantAgreement/ES/MICINN/MDM-2017-0720
Financiación: info:eu-repo/grantAgreement/ES/MINECO/BIO2015-69887-R

Tipo y forma: Article (PostPrint)

Área (Departamento): Área Química Orgánica (Dpto. Química Orgánica)