doi:10.1002/cctc.201902404engVelasco-Lozano, S.Santiago-Arcos, J.Mayoral, J.A.López-Gallego, F.Co-immobilization and Colocalization of Multi-Enzyme Systems for the Cell-Free Biosynthesis of AminoalcoholsART-2020-117956The manufacturing of aminoalcohols is an appealing transformation for industrial biocatalysis due to their high value as building blocks in synthetic chemistry. By co-immobilizing dehydrogenases, ¿-transaminases and oxidases on porous carriers, we fabricated and characterized a multi-functional heterogeneous biocatalyst that was further applied to the cell-free biosynthesis of amines and aminoalcohols from alcohols and diols, respectively. This immobilized cascade integrates both redox cofactor and amine donor recycling systems into the amino alcohol biosynthesis. Spatial co-localization of the multi-enzyme system significantly increases the cofactor recycling efficiency, the system productivity and the product conversion. Using this multi-functional heterogeneous biocatalyst, we achieved 80 % conversion of alcohols into amines and accessed a palette of up to 5 aminoalcohols starting from their corresponding diols in one-pot. The results herein presented contribute to understanding the effects of the confinement of co-immobilized enzymes on the cascade processes.2020http://zaguan.unizar.es/record/10153610.1002/cctc.201902404http://zaguan.unizar.es/record/101536oai:zaguan.unizar.es:101536info:eu-repo/grantAgreement/ES/DGA/E37-17Rinfo:eu-repo/grantAgreement/ES/DGA-FEDER/Construyendo Europa desde Aragóninfo:eu-repo/grantAgreement/EC/Era-CoBiotech/HOMBIOCAT/PCI2018-092984info:eu-repo/grantAgreement/ES/MICINN/MDM-2017-0720info:eu-repo/grantAgreement/ES/MINECO/BIO2015-69887-RChemCatChem 12, 11 (2020), 3030-3041All rights reservedhttp://www.europeana.eu/rights/rr-f/info:eu-repo/semantics/openAccess