101536 20220405150421.0 doi 10.1002/cctc.201902404 sideral 117956 ART-2020-117956 eng Velasco-Lozano, S. (orcid)0000-0001-9478-6750 Co-immobilization and Colocalization of Multi-Enzyme Systems for the Cell-Free Biosynthesis of Aminoalcohols 2020 Access copy available to the general public Unrestricted The manufacturing of aminoalcohols is an appealing transformation for industrial biocatalysis due to their high value as building blocks in synthetic chemistry. By co-immobilizing dehydrogenases, ¿-transaminases and oxidases on porous carriers, we fabricated and characterized a multi-functional heterogeneous biocatalyst that was further applied to the cell-free biosynthesis of amines and aminoalcohols from alcohols and diols, respectively. This immobilized cascade integrates both redox cofactor and amine donor recycling systems into the amino alcohol biosynthesis. Spatial co-localization of the multi-enzyme system significantly increases the cofactor recycling efficiency, the system productivity and the product conversion. Using this multi-functional heterogeneous biocatalyst, we achieved 80 % conversion of alcohols into amines and accessed a palette of up to 5 aminoalcohols starting from their corresponding diols in one-pot. The results herein presented contribute to understanding the effects of the confinement of co-immobilized enzymes on the cascade processes. info:eu-repo/grantAgreement/ES/DGA/E37-17R info:eu-repo/grantAgreement/ES/DGA-FEDER/Construyendo Europa desde Aragón info:eu-repo/grantAgreement/EC/Era-CoBiotech/HOMBIOCAT/PCI2018-092984 info:eu-repo/grantAgreement/ES/MICINN/MDM-2017-0720 info:eu-repo/grantAgreement/ES/MINECO/BIO2015-69887-R info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 5.686 2020 CHEMISTRY, PHYSICAL 50 / 162 = 0.309 2020 Q2 T1 1.497 2020 Catalysis 2020 Q1 Physical and Theoretical Chemistry 2020 Q1 Organic Chemistry 2020 Q1 Inorganic Chemistry 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Santiago-Arcos, J. Mayoral, J.A. Universidad de Zaragoza (orcid)0000-0003-1570-4257 López-Gallego, F. 2013 765 Universidad de Zaragoza Dpto. Química Orgánica Área Química Orgánica 12, 11 (2020), 3030-3041 ChemCatChem ChemCatChem 1867-3880 1053207 http://zaguan.unizar.es/record/101536/files/texto_completo.pdf Postprint 1099002 http://zaguan.unizar.es/record/101536/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:101536 articulos driver 2022-04-05-14:40:10 ARTICLE