101576 20231108202300.0 doi 10.3390/biology10030208 sideral 123857 ART-2021-123857 eng Santiago, Llipsy Determination of the Concentration of IgG against the Spike Receptor-Binding Domain That Predicts the Viral Neutralizing Activity of Convalescent Plasma and Serum against SARS-CoV-2 2021 Access copy available to the general public Unrestricted Several hundred millions of people have been diagnosed of coronavirus disease 2019 (COVID-19), causing millions of deaths and a high socioeconomic burden. SARS-CoV-2, the causative agent of COVID-19, induces both specific T- and B-cell responses, being antibodies against the virus detected a few days after infection. Passive immunization with hyperimmune plasma from convalescent patients has been proposed as a potentially useful treatment for COVID-19. Using an in-house quantitative ELISA test, we found that plasma from 177 convalescent donors contained IgG antibodies specific to the spike receptor-binding domain (RBD) of SARS-CoV-2, although at very different concentrations which correlated with previous disease severity and gender. Anti-RBD IgG plasma concentrations significantly correlated with the plasma viral neutralizing activity (VN) against SARS-CoV-2 in vitro. Similar results were found using an independent cohort of serum from 168 convalescent health workers. These results validate an in-house RBD IgG ELISA test in a large cohort of COVID-19 convalescent patients and indicate that plasma from all convalescent donors does not contain a high enough amount of anti-SARS-CoV-2-RBD neutralizing IgG to prevent SARS-CoV-2 infection in vitro. The use of quantitative anti-RBD IgG detection systems might help to predict the efficacy of the passive immunization using plasma from patients recovered from SARS-CoV-2. info:eu-repo/grantAgreement/ES/DGA-FEDER/B29-17R info:eu-repo/grantAgreement/ES/DGA-FEDER/E34-R17 info:eu-repo/grantAgreement/ES/DGA/LMP58-18 info:eu-repo/grantAgreement/ES/ISCIII/COV20-00308 info:eu-repo/grantAgreement/ES/MCIU-AEI/SAF2017-83120-C2-1-R info:eu-repo/grantAgreement/ES/MINECO/BFU2016-75633-P info:eu-repo/grantAgreement/ES/MINECO/CTQ2013-44367-C2-2-P info:eu-repo/grantAgreement/ES/MINECO/PID2019-105451GB-I00 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 5.168 2021 BIOLOGY 21 / 94 = 0.223 2021 Q1 T1 0.903 2021 Biochemistry, Genetics and Molecular Biology (miscellaneous) 2021 Q1 Agricultural and Biological Sciences (miscellaneous) 2021 Q1 2.8 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Uranga-Murillo, Iratxe Arias, Maykel González-Ramírez, Andrés M. Universidad de Zaragoza (orcid)0000-0002-5838-0857 Macías-León, Javier Universidad de Zaragoza (orcid)0000-0001-6815-6720 Moreo, Eduardo Universidad de Zaragoza (orcid)0000-0002-0182-201X Redrado, Sergio García-García, Ana Universidad de Zaragoza (orcid)0000-0003-3791-2997 Taleb, Víctor Universidad de Zaragoza (orcid)0000-0001-9224-5854 Lira-Navarrete, Erandi Universidad de Zaragoza Hurtado-Guerrero, Ramón (orcid)0000-0002-3122-9401 Aguilo, Nacho Universidad de Zaragoza (orcid)0000-0001-7897-9173 del Mar Encabo-Berzosa, Maria Hidalgo, Sandra Galvez, Eva M. Ramirez-Labrada, Ariel de Miguel, Diego Benito, Rafael Universidad de Zaragoza (orcid)0000-0001-5134-1006 Miranda, Patricia Fernández, Antonio Universidad de Zaragoza (orcid)0000-0002-2557-4890 Domingo, José M. Serrano, Laura Yuste, Cristina Villanueva-Saz, Sergio Universidad de Zaragoza (orcid)0000-0001-6209-4282 Paño-Pardo, José R. Universidad de Zaragoza (orcid)0000-0002-9600-8116 Pardo, Julián Universidad de Zaragoza (orcid)0000-0003-0154-0730 1011 630 Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Microbiología 1007 610 Universidad de Zaragoza Dpto. Medicina, Psiqu. y Derm. Area Medicina 1011 566 Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Inmunología 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 10, 3 (2021), 208 [14 pp.] Biology 2079-7737 1018448 http://zaguan.unizar.es/record/101576/files/texto_completo.pdf Versión publicada 3009960 http://zaguan.unizar.es/record/101576/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:101576 articulos driver 2023-11-08-20:14:28 ARTICLE