000101600 001__ 101600
000101600 005__ 20250425133128.0
000101600 0247_ $$2doi$$a10.1093/pcp/pcz094
000101600 0248_ $$2sideral$$a111399
000101600 037__ $$aART-2019-111399
000101600 041__ $$aeng
000101600 100__ $$0(orcid)0000-0001-6435-3540$$aSevilla, Emma$$uUniversidad de Zaragoza
000101600 245__ $$aRegulation by FurC in Anabaena links the oxidative stress response to photosynthetic metabolism
000101600 260__ $$c2019
000101600 5060_ $$aAccess copy available to the general public$$fUnrestricted
000101600 5203_ $$aThe FUR (Ferric Uptake Regulator) family in Anabaena sp. PCC 7120 consists of three paralogs named FurA (Fur), FurB (Zur) and FurC (PerR). furC seems to be an essential gene in the filamentous nitrogen-fixing strain Anabaena sp. PCC 7120, suggesting that it plays a fundamental role in this organism. In order to better understand the functions of FurC in Anabaena, the phenotype of a derivative strain that overexpresses this regulator (EB2770FurC) has been characterized. The furC-overexpressing variant presented alterations in growth rate, morphology and ultrastructure, as well as higher sensitivity to peroxide than Anabaena sp. PCC 7120. Interestingly, the overexpression of furC led to reduced photosynthetic O2 evolution, increased respiratory activity, and had a significant influence in the composition and efficiency of both photosystems. Comparative transcriptional analyses, together with electrophoretic mobility shift assays allowed the identification of different genes directly controlled by FurC, and involved in processes not previously related to PerR proteins, such as the cell division gene ftsZ and the major thylakoid membrane protease ftsH. The rise in the transcription of ftsH in EB2770FurC cells correlated with reduced levels of the D1 protein, which is involved in the PSII repair cycle. Deregulation of the oxidative stress response in EB2770FurC cells led to the identification of novel FurC targets involved in the response to H2O2 through different mechanisms. These results, together with the effect of furC overexpression on the composition, stability and efficiency of the photosynthetic machinery of Anabaena, disclose novel links between PerR proteins, cell division and photosynthesis in filamentous cyanobacteria.
000101600 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E35-17R$$9info:eu-repo/grantAgreement/ES/MINECO/BFU2012-31458$$9info:eu-repo/grantAgreement/ES/MINECO/BFU2016-77671-P
000101600 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000101600 590__ $$a4.062$$b2019
000101600 591__ $$aPLANT SCIENCES$$b25 / 234 = 0.107$$c2019$$dQ1$$eT1
000101600 591__ $$aCELL BIOLOGY$$b81 / 194 = 0.418$$c2019$$dQ2$$eT2
000101600 592__ $$a2.021$$b2019
000101600 593__ $$aCell Biology$$c2019$$dQ1
000101600 593__ $$aPlant Science$$c2019$$dQ1
000101600 593__ $$aPhysiology$$c2019$$dQ1
000101600 593__ $$aMedicine (miscellaneous)$$c2019$$dQ1
000101600 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/submittedVersion
000101600 700__ $$0(orcid)0000-0002-1960-2672$$aSarasa-Buisán, Cristina$$uUniversidad de Zaragoza
000101600 700__ $$0(orcid)0000-0002-0531-0943$$aGonzález, Andrés$$uUniversidad de Zaragoza
000101600 700__ $$0(orcid)0000-0002-9258-7907$$aCases, Rafael$$uUniversidad de Zaragoza
000101600 700__ $$aKufryk, Galyna
000101600 700__ $$0(orcid)0000-0002-2742-3711$$aPeleato, M. Luisa$$uUniversidad de Zaragoza
000101600 700__ $$0(orcid)0000-0001-8644-4574$$aFillat, María F.$$uUniversidad de Zaragoza
000101600 7102_ $$11002$$2412$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Fisiología Vegetal
000101600 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000101600 7102_ $$12003$$2395$$aUniversidad de Zaragoza$$bDpto. Física Materia Condensa.$$cÁrea Física Materia Condensada
000101600 773__ $$g60, 8 (2019), 1778–1789$$pPlant Cell Physiol.$$tPlant & Cell Physiology$$x1471-9053
000101600 8564_ $$s1123506$$uhttps://zaguan.unizar.es/record/101600/files/texto_completo.pdf$$yPreprint
000101600 8564_ $$s912059$$uhttps://zaguan.unizar.es/record/101600/files/texto_completo.jpg?subformat=icon$$xicon$$yPreprint
000101600 909CO $$ooai:zaguan.unizar.es:101600$$particulos$$pdriver
000101600 951__ $$a2025-04-25-13:29:44
000101600 980__ $$aARTICLE