101600 20250425133128.0 doi 10.1093/pcp/pcz094 sideral 111399 ART-2019-111399 eng Sevilla, Emma Universidad de Zaragoza (orcid)0000-0001-6435-3540 Regulation by FurC in Anabaena links the oxidative stress response to photosynthetic metabolism 2019 Access copy available to the general public Unrestricted The FUR (Ferric Uptake Regulator) family in Anabaena sp. PCC 7120 consists of three paralogs named FurA (Fur), FurB (Zur) and FurC (PerR). furC seems to be an essential gene in the filamentous nitrogen-fixing strain Anabaena sp. PCC 7120, suggesting that it plays a fundamental role in this organism. In order to better understand the functions of FurC in Anabaena, the phenotype of a derivative strain that overexpresses this regulator (EB2770FurC) has been characterized. The furC-overexpressing variant presented alterations in growth rate, morphology and ultrastructure, as well as higher sensitivity to peroxide than Anabaena sp. PCC 7120. Interestingly, the overexpression of furC led to reduced photosynthetic O2 evolution, increased respiratory activity, and had a significant influence in the composition and efficiency of both photosystems. Comparative transcriptional analyses, together with electrophoretic mobility shift assays allowed the identification of different genes directly controlled by FurC, and involved in processes not previously related to PerR proteins, such as the cell division gene ftsZ and the major thylakoid membrane protease ftsH. The rise in the transcription of ftsH in EB2770FurC cells correlated with reduced levels of the D1 protein, which is involved in the PSII repair cycle. Deregulation of the oxidative stress response in EB2770FurC cells led to the identification of novel FurC targets involved in the response to H2O2 through different mechanisms. These results, together with the effect of furC overexpression on the composition, stability and efficiency of the photosynthetic machinery of Anabaena, disclose novel links between PerR proteins, cell division and photosynthesis in filamentous cyanobacteria. info:eu-repo/grantAgreement/ES/DGA/E35-17R info:eu-repo/grantAgreement/ES/MINECO/BFU2012-31458 info:eu-repo/grantAgreement/ES/MINECO/BFU2016-77671-P info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.062 2019 PLANT SCIENCES 25 / 234 = 0.107 2019 Q1 T1 CELL BIOLOGY 81 / 194 = 0.418 2019 Q2 T2 2.021 2019 Cell Biology 2019 Q1 Plant Science 2019 Q1 Physiology 2019 Q1 Medicine (miscellaneous) 2019 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/submittedVersion Sarasa-Buisán, Cristina Universidad de Zaragoza (orcid)0000-0002-1960-2672 González, Andrés Universidad de Zaragoza (orcid)0000-0002-0531-0943 Cases, Rafael Universidad de Zaragoza (orcid)0000-0002-9258-7907 Kufryk, Galyna Peleato, M. Luisa Universidad de Zaragoza (orcid)0000-0002-2742-3711 Fillat, María F. Universidad de Zaragoza (orcid)0000-0001-8644-4574 1002 412 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Fisiología Vegetal 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 2003 395 Universidad de Zaragoza Dpto. Física Materia Condensa. Área Física Materia Condensada 60, 8 (2019), 1778–1789 Plant Cell Physiol. Plant & Cell Physiology 1471-9053 1123506 http://zaguan.unizar.es/record/101600/files/texto_completo.pdf Preprint 912059 http://zaguan.unizar.es/record/101600/files/texto_completo.jpg?subformat=icon icon Preprint oai:zaguan.unizar.es:101600 articulos driver 2025-04-25-13:29:44 ARTICLE