000101609 001__ 101609
000101609 005__ 20220111135614.0
000101609 0247_ $$2doi$$a10.1007/s00216-020-02677-7
000101609 0248_ $$2sideral$$a118204
000101609 037__ $$aART-2020-118204
000101609 041__ $$aeng
000101609 100__ $$0(orcid)0000-0002-3906-4576$$aSanz-Vicente, I.$$uUniversidad de Zaragoza
000101609 245__ $$aSmartphone-interrogated test supports for the enzymatic determination of putrescine and cadaverine in food
000101609 260__ $$c2020
000101609 5060_ $$aAccess copy available to the general public$$fUnrestricted
000101609 5203_ $$aDiamino-oxidase (DAO), horseradish peroxidase (HRP), and tetramethylbenzidine (TMB) have been immobilized into cellulose to obtain circular cellulose test supports (CCTSs) for the determination of cadaverine (Cad) and putrescine (Put). During the enzymatic reaction, TMB is oxidized and a blue spot is obtained. This color (RGB coordinates) is measured with a smartphone and a commercial application. The highest sensitivity is provided by the component R and a linear response is observed for low biogenic amine (BA) concentrations, but a second-order polynomial response better fits the experimental results for a wider concentration range. This has been successfully explained with a model developed to explain the RGB values obtained in this type of analytical system. Optimization studies enable CCTSs to be obtained for Put and Cad determination, which could be used (kept at 4 °C) for at least 45 days if a stabilizer (StabilCoat™ or StabilGuard™) is added during its synthesis. In these conditions, the R coordinate follows the model up to at least 4 × 10-4 M Put and/or Cad (both analytes give the same response). The method permits the Put and Cad determination from 5 × 10-5 M up to 4 × 10-4 M (RSD = 3%, n = 3). The CCTSs have been applied to Put + Cad determination in a tuna sample without any interference by other biogenic amines. The concentration found statistically agrees with that obtained using a HPLC-MS-validated method.
000101609 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FEDER/E25-17R$$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2016-76846-R
000101609 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000101609 590__ $$a4.142$$b2020
000101609 591__ $$aCHEMISTRY, ANALYTICAL$$b22 / 83 = 0.265$$c2020$$dQ2$$eT1
000101609 591__ $$aBIOCHEMICAL RESEARCH METHODS$$b20 / 77 = 0.26$$c2020$$dQ2$$eT1
000101609 592__ $$a0.86$$b2020
000101609 593__ $$aBiochemistry$$c2020$$dQ1
000101609 593__ $$aAnalytical Chemistry$$c2020$$dQ1
000101609 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000101609 700__ $$0(orcid)0000-0001-5254-4403$$aLópez-Molinero, Á.$$uUniversidad de Zaragoza
000101609 700__ $$0(orcid)0000-0002-7902-6005$$ade Marcos, S.$$uUniversidad de Zaragoza
000101609 700__ $$aNavarro, J.
000101609 700__ $$aCebrián, P.
000101609 700__ $$aArruego, C.
000101609 700__ $$aVisiedo, V.
000101609 700__ $$0(orcid)0000-0002-8973-5104$$aGalbán, J.$$uUniversidad de Zaragoza
000101609 700__ $$aNavarro Domínguez, Jesús Mª.
000101609 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000101609 773__ $$g412, 18 (2020), 4261–4271$$pAnal. Bioanal. Chem.$$tAnalytical and Bioanalytical Chemistry$$x1618-2642
000101609 8564_ $$s972611$$uhttps://zaguan.unizar.es/record/101609/files/texto_completo.pdf$$yPostprint
000101609 8564_ $$s2549498$$uhttps://zaguan.unizar.es/record/101609/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000101609 909CO $$ooai:zaguan.unizar.es:101609$$particulos$$pdriver
000101609 951__ $$a2022-01-11-13:53:11
000101609 980__ $$aARTICLE