101609 20220111135614.0 doi 10.1007/s00216-020-02677-7 sideral 118204 ART-2020-118204 eng Sanz-Vicente, I. Universidad de Zaragoza (orcid)0000-0002-3906-4576 Smartphone-interrogated test supports for the enzymatic determination of putrescine and cadaverine in food 2020 Access copy available to the general public Unrestricted Diamino-oxidase (DAO), horseradish peroxidase (HRP), and tetramethylbenzidine (TMB) have been immobilized into cellulose to obtain circular cellulose test supports (CCTSs) for the determination of cadaverine (Cad) and putrescine (Put). During the enzymatic reaction, TMB is oxidized and a blue spot is obtained. This color (RGB coordinates) is measured with a smartphone and a commercial application. The highest sensitivity is provided by the component R and a linear response is observed for low biogenic amine (BA) concentrations, but a second-order polynomial response better fits the experimental results for a wider concentration range. This has been successfully explained with a model developed to explain the RGB values obtained in this type of analytical system. Optimization studies enable CCTSs to be obtained for Put and Cad determination, which could be used (kept at 4 °C) for at least 45 days if a stabilizer (StabilCoat™ or StabilGuard™) is added during its synthesis. In these conditions, the R coordinate follows the model up to at least 4 × 10-4 M Put and/or Cad (both analytes give the same response). The method permits the Put and Cad determination from 5 × 10-5 M up to 4 × 10-4 M (RSD = 3%, n = 3). The CCTSs have been applied to Put + Cad determination in a tuna sample without any interference by other biogenic amines. The concentration found statistically agrees with that obtained using a HPLC-MS-validated method. info:eu-repo/grantAgreement/ES/DGA-FEDER/E25-17R info:eu-repo/grantAgreement/ES/MINECO/CTQ2016-76846-R info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 4.142 2020 CHEMISTRY, ANALYTICAL 22 / 83 = 0.265 2020 Q2 T1 BIOCHEMICAL RESEARCH METHODS 20 / 77 = 0.26 2020 Q2 T1 0.86 2020 Biochemistry 2020 Q1 Analytical Chemistry 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion López-Molinero, Á. Universidad de Zaragoza (orcid)0000-0001-5254-4403 de Marcos, S. Universidad de Zaragoza (orcid)0000-0002-7902-6005 Navarro, J. Cebrián, P. Arruego, C. Visiedo, V. Galbán, J. Universidad de Zaragoza (orcid)0000-0002-8973-5104 Navarro Domínguez, Jesús Mª. 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 412, 18 (2020), 4261–4271 Anal. Bioanal. Chem. Analytical and Bioanalytical Chemistry 1618-2642 972611 http://zaguan.unizar.es/record/101609/files/texto_completo.pdf Postprint 2549498 http://zaguan.unizar.es/record/101609/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:101609 articulos driver 2022-01-11-13:53:11 ARTICLE