000101618 001__ 101618
000101618 005__ 20231108202300.0
000101618 0247_ $$2doi$$a10.1186/s13071-021-04682-w
000101618 0248_ $$2sideral$$a123930
000101618 037__ $$aART-2021-123930
000101618 041__ $$aeng
000101618 100__ $$aAlcover, Maria Magdalena
000101618 245__ $$aA cross-sectional study of Leishmania infantum infection in stray cats in the city of Zaragoza (Spain) using serology and PCR
000101618 260__ $$c2021
000101618 5060_ $$aAccess copy available to the general public$$fUnrestricted
000101618 5203_ $$aBackground: Feline leishmaniosis is a vector-borne parasitic disease caused by Leishmania spp. Leishmania infection in dogs is prevalent in the Mediterranean basin, but in other animals, such as cats, it could also play a role in the epidemiology of the disease. Information on the geographical distribution and epidemiological features of L. infantum infection in cats is scarce, particularly in urban stray cats living in regions where canine leishmaniosis is endemic. As diagnosis can be challenging, combining different serological and molecular methods is a useful approach. Our aim was to investigate the prevalence of infection of L. infantum in apparently healthy stray cats in an endemic region of Spain (Zaragoza city) using serological and molecular methods, and to compare the results of the different techniques. Methods: The prevalence of Leishmania infection was studied in stray cats captured in urban and peri-urban areas of Zaragoza. Blood was collected from each animal for serology and molecular analysis. Three serological methods, namely the immunofluorescent antibody test (IFAT), enzyme-linked immunosorbent assay (ELISA) and western blot (WB), were used to detect L. infantum antibodies and a real-time PCR (qPCR) assay was used to detect L. infantum DNA. The results were analyzed by Fisher’s exact test and Cohen’s kappa statistic (¿) to assess the level of agreement between the diagnostic techniques. Results: Serological analysis of blood samples from 180 stray cats revealed 2.2% (4/179) Leishmania infection positivity by IFAT, 2.8% (5/179) by ELISA and 14.5% (26/179) by WB. Leishmania DNA was detected by qPCR in 5.6% (10/179) of the cats. Sixteen cats (8.9%) tested positive by only one serological technique and four tested positive by all three serological methods used. The overall rate of infected cats (calculated as the number of cats seropositive and/or qPCR positive) was 15.6%, and only two cats tested positive by all the diagnostic methods. A significant association was found between male cats and a positive qPCR result. Comparison of the techniques revealed a fair agreement in seropositivity between blood qPCR and IFAT (¿ = 0.26), blood qPCR and ELISA (¿ = 0.24), WB and ELISA (¿ = 0.37) and WB and IFAT (¿ = 0.40). The highest agreement between seropositive results was between IFAT and ELISA (¿ = 0.89), and the lowest was between blood qPCR and WB (¿ = 0.19). The prevalence of the feline leukemia virus antigen was 4.49% (8/178 cats) and that of the feline immunodeficiency virus (FIV) antibody was 6.74% (12/178), while co-infection with both retroviruses was observed in one female cat (1/178). Leishmania ELISA and IFAT seropositivity were statistically associated with FIV status by the chi-square test. Conclusions: The results obtained in this study, using serological tests and qPCR, indicate the existence of L. infantum asymptomatic infection in apparently healthy stray cats in the city of Zaragoza, an endemic area in Spain. [Figure not available: see fulltext.]
000101618 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000101618 590__ $$a4.052$$b2021
000101618 592__ $$a1.089$$b2021
000101618 594__ $$a6.4$$b2021
000101618 591__ $$aTROPICAL MEDICINE$$b3 / 24 = 0.125$$c2021$$dQ1$$eT1
000101618 593__ $$aParasitology$$c2021$$dQ1
000101618 591__ $$aPARASITOLOGY$$b8 / 39 = 0.205$$c2021$$dQ1$$eT1
000101618 593__ $$aInfectious Diseases$$c2021$$dQ1
000101618 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000101618 700__ $$aBasurco, Asier
000101618 700__ $$0(orcid)0000-0002-2557-4890$$aFernandez, Antonio$$uUniversidad de Zaragoza
000101618 700__ $$aRiera, Cristina
000101618 700__ $$aFisa, Roser
000101618 700__ $$0(orcid)0000-0002-8727-0323$$aGonzalez, Ana
000101618 700__ $$0(orcid)0000-0003-2957-1379$$aVerde, Maite$$uUniversidad de Zaragoza
000101618 700__ $$aGarrido, Ana María
000101618 700__ $$0(orcid)0000-0002-8474-2831$$aRuíz, Héctor$$uUniversidad de Zaragoza
000101618 700__ $$aYzuel, Andrés
000101618 700__ $$0(orcid)0000-0001-6209-4282$$aVillanueva-Saz, Sergio$$uUniversidad de Zaragoza
000101618 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000101618 773__ $$g14, 1 (2021), 178 [14 pp]$$pParasites & Vectors$$tParasites and Vectors$$x1756-3305
000101618 8564_ $$s1904000$$uhttps://zaguan.unizar.es/record/101618/files/texto_completo.pdf$$yVersión publicada
000101618 8564_ $$s2456991$$uhttps://zaguan.unizar.es/record/101618/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000101618 909CO $$ooai:zaguan.unizar.es:101618$$particulos$$pdriver
000101618 951__ $$a2023-11-08-20:14:16
000101618 980__ $$aARTICLE