doi:10.3390/polym13050684engAbad, MiriamMartínez-Bueno, AlejandroMendoza, GraciaArruebo, ManuelOriol, LuisSebastián, VíctorPiñol, MilagrosSupramolecular functionalizable linear–dendritic block copolymers for the preparation of nanocarriers by microfluidicsART-2021-124115Hybrid linear–dendritic block copolymers (LDBCs) having dendrons with a precise number of peripheral groups that are able to supramolecular bind functional moieties are challenging materials as versatile polymeric platforms for the preparation of functional polymeric nanocarriers. PEG2k-b-dxDAP LDBCs that are based on polyethylene glycol (PEG) as hydrophilic blocks and dendrons derived from bis-MPA having 2, 6-diacylaminopyridine (DAP) units have been efficiently synthesized by the click coupling of preformed blocks, as was demonstrated by spectroscopic techniques and mass spectrometry. Self-assembly ability was first checked by nanoprecipitation. A re-producible and fast synthesis of aggregates was accomplished by microfluidics optimizing the total flow rate and phase ratio to achieve spherical micelles and/or vesicles depending on dendron generation and experimental parameters. The morphology and size of the self-assemblies were studied by TEM, Cryogenic Transmission Electron Microscopy (cryo-TEM), and Dynamic Light Scattering (DLS). The cytotoxicity of aggregates synthesized by microfluidics and the influence on apoptosis and cell cycle evaluation was studied on four cell lines. The self-assemblies are not cytotoxic at doses below 0.4 mg mL–1. Supramolecular functionalization using thymine derivatives was explored for reversibly cross-linking the hydrophobic blocks. The results open new possibilities for their use as drug nanocarriers with a dynamic cross-linking to improve nanocarrier stability but without hindering disassembly to release molecular cargoes.2021http://zaguan.unizar.es/record/10164110.3390/polym13050684http://zaguan.unizar.es/record/101641oai:zaguan.unizar.es:101641info:eu-repo/grantAgreement/ES/DGA-FEDER/E47-17Rinfo:eu-repo/grantAgreement/ES/ISCIII-IIS/MS19-00092info:eu-repo/grantAgreement/ES/MICINN/RTI2018-099019-A-I00info:eu-repo/grantAgreement/ES/MINECO/MAT2017-84838-PPolymers 13, 5 (2021), 684 [27 pp.]byhttp://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccess