101641 20230519145441.0 doi 10.3390/polym13050684 sideral 124115 ART-2021-124115 eng Abad, Miriam Universidad de Zaragoza (orcid)0000-0002-8677-3316 Supramolecular functionalizable linear–dendritic block copolymers for the preparation of nanocarriers by microfluidics 2021 Access copy available to the general public Unrestricted Hybrid linear–dendritic block copolymers (LDBCs) having dendrons with a precise number of peripheral groups that are able to supramolecular bind functional moieties are challenging materials as versatile polymeric platforms for the preparation of functional polymeric nanocarriers. PEG2k-b-dxDAP LDBCs that are based on polyethylene glycol (PEG) as hydrophilic blocks and dendrons derived from bis-MPA having 2, 6-diacylaminopyridine (DAP) units have been efficiently synthesized by the click coupling of preformed blocks, as was demonstrated by spectroscopic techniques and mass spectrometry. Self-assembly ability was first checked by nanoprecipitation. A re-producible and fast synthesis of aggregates was accomplished by microfluidics optimizing the total flow rate and phase ratio to achieve spherical micelles and/or vesicles depending on dendron generation and experimental parameters. The morphology and size of the self-assemblies were studied by TEM, Cryogenic Transmission Electron Microscopy (cryo-TEM), and Dynamic Light Scattering (DLS). The cytotoxicity of aggregates synthesized by microfluidics and the influence on apoptosis and cell cycle evaluation was studied on four cell lines. The self-assemblies are not cytotoxic at doses below 0.4 mg mL–1. Supramolecular functionalization using thymine derivatives was explored for reversibly cross-linking the hydrophobic blocks. The results open new possibilities for their use as drug nanocarriers with a dynamic cross-linking to improve nanocarrier stability but without hindering disassembly to release molecular cargoes. info:eu-repo/grantAgreement/ES/DGA-FEDER/E47-17R info:eu-repo/grantAgreement/ES/ISCIII-IIS/MS19-00092 info:eu-repo/grantAgreement/ES/MICINN/RTI2018-099019-A-I00 info:eu-repo/grantAgreement/ES/MINECO/MAT2017-84838-P info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.967 2021 POLYMER SCIENCE 16 / 90 = 0.178 2021 Q1 T1 0.726 2021 Polymers and Plastics 2021 Q1 Chemistry (miscellaneous) 2021 Q1 5.7 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Martínez-Bueno, Alejandro (orcid)0000-0003-1807-2072 Mendoza, Gracia (orcid)0000-0003-2293-363X Arruebo, Manuel Universidad de Zaragoza (orcid)0000-0003-3165-0156 Oriol, Luis Universidad de Zaragoza (orcid)0000-0002-0922-5615 Sebastián, Víctor Universidad de Zaragoza (orcid)0000-0002-6873-5244 Piñol, Milagros Universidad de Zaragoza (orcid)0000-0001-5556-2172 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 2013 765 Universidad de Zaragoza Dpto. Química Orgánica Área Química Orgánica 13, 5 (2021), 684 [27 pp.] Polymers (Basel) Polymers 2073-4360 10830057 http://zaguan.unizar.es/record/101641/files/texto_completo.pdf Versión publicada 2751824 http://zaguan.unizar.es/record/101641/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:101641 articulos driver 2023-05-18-14:32:25 ARTICLE