Macro and microscopic visual imaging tools to investigate metal reducing bacteria in soils
Resumen: Indicator of Reduction In Soils (IRIS) technology is an important tool for identifying hydric soils, but it does not allow the user to monitor in real time. IRIS uses metal-oxide coatings on a polyvinyl chloride surface that, under anaerobic conditions, are removed to varying degrees over a 4-wk incubation period, during which time the user is not cognizant of the outcome. We document the viability of an alternative IRIS approach using clear-IRIS tubes, made from cellulose acetate butyrate, that can be continuously monitored in situ with a Wi-Fi–enabled video camera. This work shows that IRIS and clear-IRIS tubes are statistically equivalent. Manganese-oxide coated clear-IRIS tubes correlated well with IRIS tubes (r =.79) and ferrous-oxide had a high correlation (r =.97). A time-series analysis showed that rain-driven soil saturation induced IRIS metal-oxide reduction and controlled the rate. Clear-IRIS tubes enable remote sensing of metal-oxide removal over time.
Idioma: Inglés
DOI: 10.1002/saj2.20171
Año: 2021
Publicado en: SOIL SCIENCE SOCIETY OF AMERICA JOURNAL 85, 1 (2021), 184-192
ISSN: 0361-5995

Financiación: info:eu-repo/grantAgreement/ES/MINECO/PCI2018-092999
Tipo y forma: Article (Published version)

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