000102098 001__ 102098
000102098 005__ 20230519145358.0
000102098 0247_ $$2doi$$a10.3390/plants10030446
000102098 0248_ $$2sideral$$a124306
000102098 037__ $$aART-2021-124306
000102098 041__ $$aeng
000102098 100__ $$aPérez-Oliver, M.A.
000102098 245__ $$aPriming maritime pine megagametophytes during somatic embryogenesis improved plant adaptation to heat stress
000102098 260__ $$c2021
000102098 5060_ $$aAccess copy available to the general public$$fUnrestricted
000102098 5203_ $$aIn the context of global climate change, forest tree research should be addressed to provide genotypes with increased resilience to high temperature events. These improved plants can be obtained by heat priming during somatic embryogenesis (SE), which would produce an epigenetic-mediated transgenerational memory. Thereby, we applied 37 °C or 50 °C to maritime pine (Pinus pinaster) megagametophytes and the obtained embryogenic masses went through the subsequent SE phases to produce plants that were further subjected to heat stress conditions. A putative transcription factor WRKY11 was upregulated in priming-derived embryonal masses, and also in the regenerated P37 and P50 plants, suggesting its role in establishing an epigenetic memory in this plant species. In vitro-grown P50 plants also showed higher cytokinin content and SOD upregula-tion, which points to a better responsiveness to heat stress. Heat exposure of two-year-old maritime pine plants induced upregulation of HSP70 in those derived from primed embryogenic masses, that also showed better osmotic adjustment and higher increases in chlorophyll, soluble sugars and starch contents. Moreover, ¿PSII of P50 plants was less affected by heat exposure. Thus, our results suggest that priming at 50 °C at the SE induction phase is a promising strategy to improve heat resilience in maritime pine.
000102098 536__ $$9info:eu-repo/grantAgreement/ES/MICINN-FEDER/AGL2016-76143-C4-01-R
000102098 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000102098 590__ $$a4.658$$b2021
000102098 592__ $$a0.765$$b2021
000102098 594__ $$a3.6$$b2021
000102098 591__ $$aPLANT SCIENCES$$b39 / 240 = 0.162$$c2021$$dQ1$$eT1
000102098 593__ $$aPlant Science$$c2021$$dQ1
000102098 593__ $$aEcology, Evolution, Behavior and Systematics$$c2021$$dQ1
000102098 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000102098 700__ $$aHaro, J.G.
000102098 700__ $$aPavlovic, I.
000102098 700__ $$aNovák, O.
000102098 700__ $$aSegura, J.
000102098 700__ $$0(orcid)0000-0003-0582-5418$$aSales, E.$$uUniversidad de Zaragoza
000102098 700__ $$aArrillaga, I.
000102098 7102_ $$15011$$2705$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Producción Vegetal
000102098 773__ $$g10, 3 (2021), 446 [24 pp.]$$tPlants$$x2223-7747
000102098 8564_ $$s3653365$$uhttps://zaguan.unizar.es/record/102098/files/texto_completo.pdf$$yVersión publicada
000102098 8564_ $$s2697764$$uhttps://zaguan.unizar.es/record/102098/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000102098 909CO $$ooai:zaguan.unizar.es:102098$$particulos$$pdriver
000102098 951__ $$a2023-05-18-13:34:54
000102098 980__ $$aARTICLE