000102104 001__ 102104
000102104 005__ 20240104111817.0
000102104 0247_ $$2doi$$a10.3390/ani11041137
000102104 0248_ $$2sideral$$a124294
000102104 037__ $$aART-2021-124294
000102104 041__ $$aeng
000102104 100__ $$0(orcid)0000-0002-2954-1068$$aGarcía-Mendívil, Laura$$uUniversidad de Zaragoza
000102104 245__ $$aEffect of scrapie prion infection in ovine bone marrow-derived mesenchymal stem cells and ovine mesenchymal stem cell-derived neurons
000102104 260__ $$c2021
000102104 5060_ $$aAccess copy available to the general public$$fUnrestricted
000102104 5203_ $$aScrapie is a prion disease affecting sheep and goats and it is considered a prototype of transmissible spongiform encephalopathies (TSEs). Mesenchymal stem cells (MSCs) have been proposed as candidates for developing in vitro models of prion diseases. Murine MSCs are able to propagate prions after previous mouse-adaptation of prion strains and, although ovine MSCs express the cellular prion protein (PrPC), their susceptibility to prion infection has never been investigated. Here, we analyze the potential of ovine bone marrow-derived MSCs (oBM-MSCs), in growth and neurogenic conditions, to be infected by natural scrapie and propagate prion particles (PrPSc) in vitro, as well as the effect of this infection on cell viability and proliferation. Cultures were kept for 48–72 h in contact with homogenates of central nervous system (CNS) samples from scrapie or control sheep. In growth conditions, oBM-MSCs initially maintained detectable levels of PrPSc post-inoculation, as determined by Western blotting and ELISA. However, the PrPSc signal weakened and was lost over time. oBM-MSCs infected with scrapie displayed lower cell doubling and higher doubling times than those infected with control inocula. On the other hand, in neurogenic conditions, oBM-MSCs not only maintained detectable levels of PrPSc post-inoculation, as determined by ELISA, but this PrPSc signal also increased progressively over time. Finally, inoculation with CNS extracts seems to induce the proliferation of oBM-MSCs in both growth and neurogenic conditions. Our results suggest that oBM-MSCs respond to prion infection by decreasing their proliferation capacity and thus might not be permissive to prion replication, whereas ovine MSC-derived neuron-like cells seem to maintain and replicate PrPSc.
000102104 536__ $$9info:eu-repo/grantAgreement/EUR/INTERREG-POCTEFA/EFA-148-16 REDPRION$$9info:eu-repo/grantAgreement/ES/MICINN/RTI2018-098711-B-I00
000102104 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000102104 590__ $$a3.231$$b2021
000102104 592__ $$a0.61$$b2021
000102104 594__ $$a2.7$$b2021
000102104 591__ $$aVETERINARY SCIENCES$$b16 / 145 = 0.11$$c2021$$dQ1$$eT1
000102104 593__ $$aVeterinary (miscellaneous)$$c2021$$dQ1
000102104 591__ $$aAGRICULTURE, DAIRY & ANIMAL SCIENCE$$b13 / 62 = 0.21$$c2021$$dQ1$$eT1
000102104 593__ $$aAnimal Science and Zoology$$c2021$$dQ1
000102104 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000102104 700__ $$0(orcid)0000-0002-8692-1382$$aMediano, Diego R.
000102104 700__ $$0(orcid)0000-0002-6806-9990$$aHernaiz, Adelaida$$uUniversidad de Zaragoza
000102104 700__ $$0(orcid)0000-0002-5228-248X$$aSanz-Rubio, David
000102104 700__ $$0(orcid)0000-0002-8712-2275$$aVázquez, Francisco J.$$uUniversidad de Zaragoza
000102104 700__ $$0(orcid)0000-0002-1590-3347$$aMarín, Belén$$uUniversidad de Zaragoza
000102104 700__ $$0(orcid)0000-0003-2454-2114$$aLópez-Pérez, Óscar
000102104 700__ $$0(orcid)0000-0001-9075-2764$$aOtero, Alicia$$uUniversidad de Zaragoza
000102104 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, Juan J.$$uUniversidad de Zaragoza
000102104 700__ $$0(orcid)0000-0001-5740-0185$$aZaragoza, Pilar$$uUniversidad de Zaragoza
000102104 700__ $$0(orcid)0000-0003-3982-1263$$aOrdovás, Laura
000102104 700__ $$0(orcid)0000-0002-2746-3932$$aBolea, Rosa$$uUniversidad de Zaragoza
000102104 700__ $$0(orcid)0000-0001-6016-4726$$aMartín-Burriel, Inmaculada$$uUniversidad de Zaragoza
000102104 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000102104 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000102104 7102_ $$15008$$2800$$aUniversidad de Zaragoza$$bDpto. Ingeniería Electrón.Com.$$cÁrea Teoría Señal y Comunicac.
000102104 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000102104 773__ $$g11, 4 (2021), 1137 [13 pp.]$$pAnimals (Basel)$$tAnimals$$x2076-2615
000102104 8564_ $$s1992828$$uhttps://zaguan.unizar.es/record/102104/files/texto_completo.pdf$$yVersión publicada
000102104 8564_ $$s2958140$$uhttps://zaguan.unizar.es/record/102104/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000102104 909CO $$ooai:zaguan.unizar.es:102104$$particulos$$pdriver
000102104 951__ $$a2024-01-04-11:05:23
000102104 980__ $$aARTICLE