102104 20240104111817.0 doi 10.3390/ani11041137 sideral 124294 ART-2021-124294 eng García-Mendívil, Laura Universidad de Zaragoza (orcid)0000-0002-2954-1068 Effect of scrapie prion infection in ovine bone marrow-derived mesenchymal stem cells and ovine mesenchymal stem cell-derived neurons 2021 Access copy available to the general public Unrestricted Scrapie is a prion disease affecting sheep and goats and it is considered a prototype of transmissible spongiform encephalopathies (TSEs). Mesenchymal stem cells (MSCs) have been proposed as candidates for developing in vitro models of prion diseases. Murine MSCs are able to propagate prions after previous mouse-adaptation of prion strains and, although ovine MSCs express the cellular prion protein (PrPC), their susceptibility to prion infection has never been investigated. Here, we analyze the potential of ovine bone marrow-derived MSCs (oBM-MSCs), in growth and neurogenic conditions, to be infected by natural scrapie and propagate prion particles (PrPSc) in vitro, as well as the effect of this infection on cell viability and proliferation. Cultures were kept for 48–72 h in contact with homogenates of central nervous system (CNS) samples from scrapie or control sheep. In growth conditions, oBM-MSCs initially maintained detectable levels of PrPSc post-inoculation, as determined by Western blotting and ELISA. However, the PrPSc signal weakened and was lost over time. oBM-MSCs infected with scrapie displayed lower cell doubling and higher doubling times than those infected with control inocula. On the other hand, in neurogenic conditions, oBM-MSCs not only maintained detectable levels of PrPSc post-inoculation, as determined by ELISA, but this PrPSc signal also increased progressively over time. Finally, inoculation with CNS extracts seems to induce the proliferation of oBM-MSCs in both growth and neurogenic conditions. Our results suggest that oBM-MSCs respond to prion infection by decreasing their proliferation capacity and thus might not be permissive to prion replication, whereas ovine MSC-derived neuron-like cells seem to maintain and replicate PrPSc. info:eu-repo/grantAgreement/EUR/INTERREG-POCTEFA/EFA-148-16 REDPRION info:eu-repo/grantAgreement/ES/MICINN/RTI2018-098711-B-I00 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.231 2021 VETERINARY SCIENCES 16 / 145 = 0.11 2021 Q1 T1 AGRICULTURE, DAIRY & ANIMAL SCIENCE 13 / 62 = 0.21 2021 Q1 T1 0.61 2021 Veterinary (miscellaneous) 2021 Q1 Animal Science and Zoology 2021 Q1 2.7 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Mediano, Diego R. (orcid)0000-0002-8692-1382 Hernaiz, Adelaida Universidad de Zaragoza (orcid)0000-0002-6806-9990 Sanz-Rubio, David (orcid)0000-0002-5228-248X Vázquez, Francisco J. Universidad de Zaragoza (orcid)0000-0002-8712-2275 Marín, Belén Universidad de Zaragoza (orcid)0000-0002-1590-3347 López-Pérez, Óscar (orcid)0000-0003-2454-2114 Otero, Alicia Universidad de Zaragoza (orcid)0000-0001-9075-2764 Badiola, Juan J. Universidad de Zaragoza (orcid)0000-0002-7173-7216 Zaragoza, Pilar Universidad de Zaragoza (orcid)0000-0001-5740-0185 Ordovás, Laura (orcid)0000-0003-3982-1263 Bolea, Rosa Universidad de Zaragoza (orcid)0000-0002-2746-3932 Martín-Burriel, Inmaculada Universidad de Zaragoza (orcid)0000-0001-6016-4726 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 5008 800 Universidad de Zaragoza Dpto. Ingeniería Electrón.Com. Área Teoría Señal y Comunicac. 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 11, 4 (2021), 1137 [13 pp.] Animals (Basel) Animals 2076-2615 1992828 http://zaguan.unizar.es/record/102104/files/texto_completo.pdf Versión publicada 2958140 http://zaguan.unizar.es/record/102104/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:102104 articulos driver 2024-01-04-11:05:23 ARTICLE