000102117 001__ 102117
000102117 005__ 20230519145405.0
000102117 0247_ $$2doi$$a10.3389/fpls.2021.622578
000102117 0248_ $$2sideral$$a124340
000102117 037__ $$aART-2021-124340
000102117 041__ $$aeng
000102117 100__ $$aAmri, R.
000102117 245__ $$aBiochemical Characterization and Differential Expression of PAL Genes Associated With “Translocated” Peach/Plum Graft-Incompatibility
000102117 260__ $$c2021
000102117 5060_ $$aAccess copy available to the general public$$fUnrestricted
000102117 5203_ $$aGrafting is an ancient plant propagation technique widely used in horticultural crops, particularly in fruit trees. However, the involvement of two different species in grafting may lead to lack of affinity and severe disorders between the graft components, known as graft-incompatibility. This complex agronomic trait is traditionally classified into two categories: “localized” (weak graft unions with breaks in cambial and vascular continuity at the graft interface and absence of visual symptoms in scion leaves and shoots) and “translocated” (degeneration of the sieve tubes and phloem companion cells at the graft interface causing translocation problems in neighboring tissues, and reddening/yellowing of scion leaves). Over the decades, more attention has been given to the different mechanisms underlying the “localized” type of graft-incompatibility; whereas the phenylpropanoid-derived compounds and the differential gene expression associated with the “translocated” graft-incompatibility remain unstudied. Therefore, the aim of this study was to shed light on the biochemical and molecular mechanisms involved in the typical “translocated” graft-incompatibility of peach/plum graft-combinations. In this study, the “Summergrand” (SG) nectarine cultivar was budded on two plum rootstocks: “Adara” and “Damas GF 1869”. “Translocated” symptoms of incompatibility were shown and biochemically characterized in the case of “SG/Damas GF 1869” graft-combination, 3 years after grafting. Non-structural carbohydrates (soluble sugars and starch), phenolic compounds and antioxidant activity, were significantly enhanced in the incompatible graft-combination scion. Similarly, the enzymatic activities of the antioxidant enzyme peroxidase, the phenylalanine ammonia-lyase (PAL) and polyphenol oxidase involved in the phenylpropanoid pathway were significantly affected by the incompatible rootstock “Damas GF 1869”, inducing higher activities in the scion than those induced by the compatible rootstock “Adara”. In addition, a positive and strong correlation was obtained between total phenol content, antioxidant capacity and the expression of the key genes involved in the phenylpropanoid pathway, PAL1 and PAL2. Regarding the “SG/Adara” graft-combination, there were neither external symptoms of “translocated” incompatibility nor significant differences in the biochemical and molecular parameters between scion and rootstock, proving it to be a compatible combination. The differential expression of PAL genes together with the biochemical factors cited above could be good markers for the “translocated” peach/plum graft-incompatibility. ©
000102117 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A44$$9info:eu-repo/grantAgreement/ES/DGA/T07-17R$$9info:eu-repo/grantAgreement/ES/MICINN-FEDER/RFP2015-00019$$9info:eu-repo/grantAgreement/ES/MICINN-FEDER/RTI2018-094176-R-C33
000102117 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000102117 590__ $$a6.627$$b2021
000102117 592__ $$a1.359$$b2021
000102117 594__ $$a8.0$$b2021
000102117 591__ $$aPLANT SCIENCES$$b20 / 240 = 0.083$$c2021$$dQ1$$eT1
000102117 593__ $$aPlant Science$$c2021$$dQ1
000102117 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000102117 700__ $$aFont i Forcada, C.
000102117 700__ $$aGiménez, R.
000102117 700__ $$0(orcid)0000-0002-4988-9467$$aPina, A.
000102117 700__ $$aMoreno, M.Á.
000102117 773__ $$g12 (2021), 622578 [14 pp]$$pFront. plant sci.$$tFrontiers in Plant Science$$x1664-462X
000102117 8564_ $$s4393382$$uhttps://zaguan.unizar.es/record/102117/files/texto_completo.pdf$$yVersión publicada
000102117 8564_ $$s2488983$$uhttps://zaguan.unizar.es/record/102117/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000102117 909CO $$ooai:zaguan.unizar.es:102117$$particulos$$pdriver
000102117 951__ $$a2023-05-18-13:46:38
000102117 980__ $$aARTICLE