000102172 001__ 102172
000102172 005__ 20230519145441.0
000102172 0247_ $$2doi$$a10.3390/ijms22052714
000102172 0248_ $$2sideral$$a124123
000102172 037__ $$aART-2021-124123
000102172 041__ $$aeng
000102172 100__ $$0(orcid)0000-0002-7037-6316$$aBarrio, Tomás
000102172 245__ $$aEvidence of p75 neurotrophin receptor involvement in the central nervous system pathogenesis of classical scrapie in sheep and a transgenic mouse model
000102172 260__ $$c2021
000102172 5060_ $$aAccess copy available to the general public$$fUnrestricted
000102172 5203_ $$aNeurotrophins constitute a group of growth factor that exerts important functions in the nervous system of vertebrates. They act through two classes of transmembrane receptors: tyrosine-kinase receptors and the p75 neurotrophin receptor (p75NTR ). The activation of p75NTR can favor cell survival or apoptosis depending on diverse factors. Several studies evidenced a link between p75NTR and the pathogenesis of prion diseases. In this study, we investigated the distribution of several neurotrophins and their receptors, including p75NTR, in the brain of naturally scrapie-affected sheep and experimentally infected ovinized transgenic mice and its correlation with other markers of prion disease. No evident changes in infected mice or sheep were observed regarding neurotrophins and their receptors except for the immunohistochemistry against p75NTR . Infected mice showed higher abundance of p75NTR immunostained cells than their non-infected counterparts. The astrocytic labeling correlated with other neuropathological alterations of prion disease. Confocal microscopy demonstrated the co-localization of p75NTR and the astrocytic marker GFAP, suggesting an involvement of astrocytes in p75NTR-mediated neurodegeneration. In contrast, p75NTR staining in sheep lacked astrocytic labeling. However, digital image analyses revealed increased labeling intensities in preclinical sheep compared with non-infected and terminal sheep in several brain nuclei. This suggests that this receptor is overexpressed in early stages of prion-related neurodegeneration in sheep. Our results confirm a role of p75NTR in the pathogenesis of classical ovine scrapie in both the natural host and in an experimental transgenic mouse model.
000102172 536__ $$9info:eu-repo/grantAgreement/ES/MEC/FPU14-04348$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2015-65560-R
000102172 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000102172 590__ $$a6.208$$b2021
000102172 592__ $$a1.176$$b2021
000102172 594__ $$a6.9$$b2021
000102172 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b69 / 297 = 0.232$$c2021$$dQ1$$eT1
000102172 593__ $$aComputer Science Applications$$c2021$$dQ1
000102172 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b50 / 180 = 0.278$$c2021$$dQ2$$eT1
000102172 593__ $$aInorganic Chemistry$$c2021$$dQ1
000102172 593__ $$aSpectroscopy$$c2021$$dQ1
000102172 593__ $$aOrganic Chemistry$$c2021$$dQ1
000102172 593__ $$aPhysical and Theoretical Chemistry$$c2021$$dQ1
000102172 593__ $$aMolecular Biology$$c2021$$dQ1
000102172 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000102172 700__ $$aVidal, Enric
000102172 700__ $$0(orcid)0000-0002-0388-9429$$aBetancor, Marina$$uUniversidad de Zaragoza
000102172 700__ $$0(orcid)0000-0001-9075-2764$$aOtero, Alicia$$uUniversidad de Zaragoza
000102172 700__ $$0(orcid)0000-0001-6016-4726$$aMartín-Burriel, Inmaculada$$uUniversidad de Zaragoza
000102172 700__ $$0(orcid)0000-0002-2787-9671$$aMonzón, Marta$$uUniversidad de Zaragoza
000102172 700__ $$0(orcid)0000-0002-7453-1766$$aMonleón, Eva$$uUniversidad de Zaragoza
000102172 700__ $$aPumarola, Martí
000102172 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, Juan José$$uUniversidad de Zaragoza
000102172 700__ $$0(orcid)0000-0002-2746-3932$$aBolea, Rosa$$uUniversidad de Zaragoza
000102172 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000102172 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000102172 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000102172 773__ $$g22, 5 (2021), 2714 [18 pp.]$$pInt. j. mol. sci.$$tInternational Journal of Molecular Sciences$$x1661-6596
000102172 8564_ $$s740968$$uhttps://zaguan.unizar.es/record/102172/files/texto_completo.pdf$$yVersión publicada
000102172 8564_ $$s2849075$$uhttps://zaguan.unizar.es/record/102172/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000102172 909CO $$ooai:zaguan.unizar.es:102172$$particulos$$pdriver
000102172 951__ $$a2023-05-18-14:33:08
000102172 980__ $$aARTICLE