000102175 001__ 102175
000102175 005__ 20250109150035.0
000102175 0247_ $$2doi$$a10.3390/ijms22052523
000102175 0248_ $$2sideral$$a124129
000102175 037__ $$aART-2021-124129
000102175 041__ $$aeng
000102175 100__ $$0(orcid)0000-0003-2436-7205$$aMoreno-García, L.$$uUniversidad de Zaragoza
000102175 245__ $$aInflammasome in ALS skeletal muscle: NLRP3 as a potential biomarker
000102175 260__ $$c2021
000102175 5060_ $$aAccess copy available to the general public$$fUnrestricted
000102175 5203_ $$aSince NLRP3 inflammasome plays a pivotal role in several neurodegenerative disorders, we hypothesized that levels of inflammasome components could help in diagnosis or prognosis of amyotrophic lateral sclerosis (ALS). Gene and protein expression was assayed by RT-PCR and Western blot. Spearman’s correlation coefficient was used to determine the linear correlation of transcriptional expression levels with longevity throughout disease progression in mice models. Kaplan-Meier analysis was performed to evaluate MCC950 effects (NLRP3 inhibitor) on lifespan of SOD1G93A mice. The results showed significant alterations in NLRP3 inflammasome gene and protein levels in the skeletal muscle of SOD1G93A mice. Spearman’s correlation coefficient revealed a positive association between Nlrp3 transcriptional levels in skeletal muscle and longevity of SOD1G93A mice (r = 0.506; p = 0.027). Accordingly, NLRP3 inactivation with MCC950 decreased the lifespan of mice. Furthermore, NLRP3 mRNA levels were significantly elevated in the blood of ALS patients compared to healthy controls (p = 0.03). In conclusion, NLRP3 could be involved in skeletal muscle pathogenesis of ALS, either through inflammasome or independently, and may play a dual role during disease progression. NLRP3 gene expression levels could be used as a biomarker to improve diagnosis and prognosis in skeletal muscle from animal models and also to support diagnosis in clinical practice with the blood of ALS patients.
000102175 536__ $$9info:eu-repo/grantAgreement/ES/FIS-FEDER/PI17-00949$$9info:eu-repo/grantAgreement/ES/UZ/2018-BIO-03
000102175 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000102175 590__ $$a6.208$$b2021
000102175 592__ $$a1.176$$b2021
000102175 594__ $$a6.9$$b2021
000102175 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b69 / 297 = 0.232$$c2021$$dQ1$$eT1
000102175 593__ $$aComputer Science Applications$$c2021$$dQ1
000102175 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b50 / 179 = 0.279$$c2021$$dQ2$$eT1
000102175 593__ $$aInorganic Chemistry$$c2021$$dQ1
000102175 593__ $$aSpectroscopy$$c2021$$dQ1
000102175 593__ $$aOrganic Chemistry$$c2021$$dQ1
000102175 593__ $$aPhysical and Theoretical Chemistry$$c2021$$dQ1
000102175 593__ $$aMolecular Biology$$c2021$$dQ1
000102175 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000102175 700__ $$0(orcid)0000-0001-5981-5448$$aMiana-Mena, F.J.$$uUniversidad de Zaragoza
000102175 700__ $$0(orcid)0000-0002-7277-4318$$aMoreno-Martínez, L.$$uUniversidad de Zaragoza
000102175 700__ $$0(orcid)0000-0002-7955-7164$$ade la Torre, M.$$uUniversidad de Zaragoza
000102175 700__ $$aLunetta, C.
000102175 700__ $$aTarlarini, C.
000102175 700__ $$0(orcid)0000-0001-5740-0185$$aZaragoza, P.$$uUniversidad de Zaragoza
000102175 700__ $$0(orcid)0000-0001-5193-7782$$aCalvo, A.C.$$uUniversidad de Zaragoza
000102175 700__ $$0(orcid)0000-0001-5687-6704$$aOsta, R.$$uUniversidad de Zaragoza
000102175 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000102175 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología
000102175 7102_ $$11012$$2315$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Farmacología
000102175 773__ $$g22, 5 (2021), 2523 [15 pp.]$$pInt. j. mol. sci.$$tInternational Journal of Molecular Sciences$$x1661-6596
000102175 8564_ $$s1520894$$uhttps://zaguan.unizar.es/record/102175/files/texto_completo.pdf$$yVersión publicada
000102175 8564_ $$s2817825$$uhttps://zaguan.unizar.es/record/102175/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000102175 909CO $$ooai:zaguan.unizar.es:102175$$particulos$$pdriver
000102175 951__ $$a2025-01-09-14:59:01
000102175 980__ $$aARTICLE