102211 20220908120515.0 doi 10.1016/j.ctarc.2021.100355 sideral 124382 ART-2021-124382 eng Al-Wasaby, S. In vivo potential of recombinant granulysin against human melanoma 2021 Access copy available to the general public Unrestricted 9-kDa granulysin is a protein expressed into the granules of human cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. It has been shown to exert cytolysis on microbes and tumors. We showed previously that 9-kDa granulysin exerted cell death by apoptosis in vitro on hematological tumor cell lines and also on cells from B-cell chronic lymphocytic leukemia (B-CLL) patients. In addition, we have shown the anti-tumor efficiency of granulysin as a single agent in two in vivo models of human tumor development in athymic mice, the MDA-MB-231 mammary adenocarcinoma and the NCI-H929 multiple myeloma, without signs of overt secondary effects by itself. In this work, we have tested recombinant 9-kDa granulysin in an in vivo and especially aggressive model of melanoma development, xenografted UACC62 cells in athymic mice. Recombinant granulysin was administered once UACC62-derived tumors were detectable and it substantially retarded the in vivo development of this aggressive tumor. We could also detect apoptosis induction and increased NK cell infiltration inside granulysin-treated tumor tissues. These observations are especially interesting given the possibility of treating melanoma by intra-tumor injection. info:eu-repo/grantAgreement/ES/DGA/B31-17R info:eu-repo/grantAgreement/ES/MINECO/SAF2016-76338-R info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 0.52 2021 Oncology 2021 Q3 Cancer Research 2021 Q3 1.5 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Guerrero-Ochoa, P. Universidad de Zaragoza (orcid)0000-0002-1657-4792 Ibáñez-Pérez, R. Universidad de Zaragoza Soler, R. Universidad de Zaragoza Conde, B. Universidad de Zaragoza (orcid)0000-0003-4115-9766 Martínez-Lostao, L. Universidad de Zaragoza (orcid)0000-0003-3043-147X Anel, A. Universidad de Zaragoza (orcid)0000-0002-5175-8394 1003 443 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Histología 1011 566 Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Inmunología 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 1002 050 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Biología Celular 27 (2021), 100355 [7 pp.] Cancer Treatment and Research Communications 2468-2942 7920905 http://zaguan.unizar.es/record/102211/files/texto_completo.pdf Versión publicada 2558529 http://zaguan.unizar.es/record/102211/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:102211 articulos driver 2022-09-08-11:54:50 ARTICLE