000106169 001__ 106169
000106169 005__ 20230519145517.0
000106169 0247_ $$2doi$$a10.3389/fmicb.2021.621737
000106169 0248_ $$2sideral$$a124091
000106169 037__ $$aART-2021-124091
000106169 041__ $$aeng
000106169 100__ $$aBacian, C.
000106169 245__ $$aLongitudinal Study of Total and Pathogenic Vibrio parahaemolyticus (tdh+ and/or trh+) in Two Natural Extraction Areas of Mytilus chilensis in Southern Chile
000106169 260__ $$c2021
000106169 5060_ $$aAccess copy available to the general public$$fUnrestricted
000106169 5203_ $$aVibrio parahaemolyticus is the leading cause of seafood-associated bacterial gastroenteritis worldwide. Although different studies have focused on its pattern of variation over time, knowledge about the environmental factors driving the dynamics of this pathogen, within the Chilean territory, is still lacking. This study determined the prevalence of total and pathogenic V. parahaemolyticus strains (tdh and/or trh genes) in mussels (Mytilus chilensis) collected from two natural growing areas between 2017 and 2018, using selective agar and PCR analysis. V. parahaemolyticus was detected in 45.6% (93/204) of pooled samples from the Valdivia River Estuary. The pathogenic strains carrying the tdh and/or trh gene were detected in 11.8% (24/204): tdh in 9.8% (20/204), trh in 0.5% (1/204), and 1.5% (3/204) presented both genes. In Reloncaví Fjord, V. parahaemolyticus was detected in 14.4% (30/209) of the samples, pathogenic V. parahaemolyticus carrying the trh gene was detected in 0.5% (1/209) of the samples, while the tdh gene was not detected in the samples from this area. The total count of mauve-purple colonies typical of V. parahaemolyticus on CHROMagar was positively associated by multivariate analysis with area, water temperature, and salinity. Similarly, V. parahaemolyticus detection rates by PCR had a positive correlation with the area and water temperature. The chances of detecting total V. parahaemolyticus in the Valdivia River Estuary are significantly higher than in the Reloncaví Fjord, but inversely, during spring-summer months, the interaction factor between the area and temperature indicated that the chances of detecting V. parahaemolyticus are higher in the Reloncaví Fjord. Interestingly, this period coincides with the season when commercial and natural-growing shellfish are harvested. On the other hand, pathogenic V. parahaemolyticus tdh+ was significantly correlated with an increase of water temperature. These environmental parameters could be used to trigger a warning on potential hazard, which would influence human health and economic losses in aquaculture systems. ©
000106169 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000106169 590__ $$a6.064$$b2021
000106169 592__ $$a1.314$$b2021
000106169 594__ $$a8.2$$b2021
000106169 591__ $$aMICROBIOLOGY$$b34 / 138 = 0.246$$c2021$$dQ1$$eT1
000106169 593__ $$aMicrobiology (medical)$$c2021$$dQ1
000106169 593__ $$aMicrobiology$$c2021$$dQ1
000106169 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000106169 700__ $$aVerdugo, C.
000106169 700__ $$aGarcía, K.
000106169 700__ $$aPerez-Larruscain, J.
000106169 700__ $$0(orcid)0000-0002-1204-4356$$ade Blas, I.$$uUniversidad de Zaragoza
000106169 700__ $$aCachicas, V.
000106169 700__ $$aLopez-Joven, C.
000106169 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000106169 773__ $$g12 (2021), 621737 [11 pp]$$pFront. microbiol.$$tFrontiers in Microbiology$$x1664-302X
000106169 8564_ $$s2665857$$uhttps://zaguan.unizar.es/record/106169/files/texto_completo.pdf$$yVersión publicada
000106169 8564_ $$s2587440$$uhttps://zaguan.unizar.es/record/106169/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000106169 909CO $$ooai:zaguan.unizar.es:106169$$particulos$$pdriver
000106169 951__ $$a2023-05-18-15:18:31
000106169 980__ $$aARTICLE