000106251 001__ 106251
000106251 005__ 20210902121830.0
000106251 0247_ $$2doi$$a10.3791/61440
000106251 0248_ $$2sideral$$a119197
000106251 037__ $$aART-2020-119197
000106251 041__ $$aeng
000106251 100__ $$aMedina-Lozano, Inés
000106251 245__ $$aImproved UPLC-UV method for the quantification of vitamin C in lettuce varieties (Lactuca sativa L.) and crop wild relatives (Lactuca spp.)
000106251 260__ $$c2020
000106251 5060_ $$aAccess copy available to the general public$$fUnrestricted
000106251 5203_ $$aVitamins, especially vitamin C, are important micronutrients found in fruits and vegetables. Vitamin C is also a major contributor to their antioxidant capacity. Lettuce is one of the most popular vegetables among consumers worldwide. An accurate protocol to measure vitamin C content in lettuce and other related species is crucial. We describe here a method using the ultra-high-performance liquid chromatography-ultraviolet (UPLC-UV) technique, in which sample preparation, vitamin extraction and chromatography conditions were optimized. Samples were collected to represent the entire plant, frozen at-80 °C and lyophilized to prevent undesirable oxidation and make their manipulation easier. The extraction of vitamin C was carried out in acidic media, which also contributed to its stability. As vitamin C can be present in two different interconvertible forms, ascorbic acid (AA) and dehydroascorbic acid (DHAA), both compounds should be measured for accurate quantification. The DHAA was quantified indirectly after its reduction to AA because AA shows a higher absorptivity than DHAA in the UV range of the spectrum. From the same extract, two measurements were carried out, one before and one after that reduction reaction. In the first case, we were quantifying the AA content, and in the second one, we quantified the sum of AA and DHAA (TAA: total ascorbic acid) in the form of AA. Then, DHAA quantity was indirectly obtained by subtracting AA coming from the first measurement from TAA. They were determined by UPLC-UV, using a commercial AA standard to build a calibration curve and optimizing the chromatographic procedure, to obtain AA peaks that were completely resolved in a short time. This protocol could be easily extrapolated to any other plant material with slight or no changes. Its accuracy revealed statistically significant differences otherwise unperceived. Other strengths and limitations are discussed more in depth in the manuscript.
000106251 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A12-17R$$9info:eu-repo/grantAgreement/ES/DGA/A14-17R$$9info:eu-repo/grantAgreement/ES/DGA/LMP164-18$$9info:eu-repo/grantAgreement/ES/INIA/RTA2017-00093-00-00
000106251 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000106251 590__ $$a1.355$$b2020
000106251 591__ $$aMULTIDISCIPLINARY SCIENCES$$b49 / 73 = 0.671$$c2020$$dQ3$$eT3
000106251 592__ $$a0.595$$b2020
000106251 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2020$$dQ2
000106251 593__ $$aNeuroscience (miscellaneous)$$c2020$$dQ2
000106251 593__ $$aChemical Engineering (miscellaneous)$$c2020$$dQ2
000106251 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000106251 700__ $$0(orcid)0000-0001-6673-489X$$aBertolín, Juan Ramón
000106251 700__ $$0(orcid)0000-0003-2368-9878$$aZufiaurre, Raquel$$uUniversidad de Zaragoza
000106251 700__ $$0(orcid)0000-0001-7297-1699$$aDíaz, Aurora$$uUniversidad de Zaragoza
000106251 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000106251 7102_ $$15011$$2705$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Producción Vegetal
000106251 773__ $$g160 (2020), e61440 1-16$$pJ. vis. exp.$$tJournal of visualized experiments : JoVE$$x1940-087X
000106251 8564_ $$s407486$$uhttps://zaguan.unizar.es/record/106251/files/texto_completo.pdf$$yVersión publicada
000106251 8564_ $$s2062825$$uhttps://zaguan.unizar.es/record/106251/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000106251 909CO $$ooai:zaguan.unizar.es:106251$$particulos$$pdriver
000106251 951__ $$a2021-09-02-10:15:05
000106251 980__ $$aARTICLE