doi:10.1021/acs.nanolett.0c02163engPiñol, R.Zeler, J.Brites, C.D.S.Gu, Y.Téllez, P.Carneiro Neto, A.N.da Silva, T.E.Moreno-Loshuertos, R.Fernandez-Silva, P.Gallego, A.I.Martinez-Lostao, L.Martínez, A.Carlos, L.D.Millán, A.Real-Time Intracellular Temperature Imaging Using Lanthanide-Bearing Polymeric MicellesART-2020-120307Measurement of thermogenesis in individual cells is a remarkable challenge due to the complexity of the biochemical environment (such as pH and ionic strength) and to the rapid and yet not well-understood heat transfer mechanisms throughout the cell. Here, we present a unique system for intracellular temperature mapping in a fluorescence microscope (uncertainty of 0.2 K) using rationally designed luminescent Ln3+-bearing polymeric micellar probes (Ln = Sm, Eu) incubated in breast cancer MDA-MB468 cells. Two-dimensional (2D) thermal images recorded increasing the temperature of the cells culture medium between 296 and 304 K shows inhomogeneous intracellular temperature progressions up to ~20 degrees and subcellular gradients of ~5 degrees between the nucleolus and the rest of the cell, illustrating the thermogenic activity of the different organelles and highlighting the potential of this tool to study intracellular processes.2020http://zaguan.unizar.es/record/10658810.1021/acs.nanolett.0c02163http://zaguan.unizar.es/record/106588oai:zaguan.unizar.es:106588info:eu-repo/grantAgreement/ES/DGA/E11-17Rinfo:eu-repo/grantAgreement/EC/H2020/801305/EU/Nanoparticles-based 2D thermal bioimaging technologies/NanoTBTechThis project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 801305-NanoTBTechinfo:eu-repo/grantAgreement/EC/H2020/829162/EU/Redesigning biocatalysis: Thermal-tuning of one-pot multienzymatic cascades by nanoactuation/HOTZYMESThis project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 829162-HOTZYMESinfo:eu-repo/grantAgreement/ES/MINECO-FEDER/PGC2018-095795-B-I00Nano Letters 20, 9 (2020), 6466-6472All rights reservedhttp://www.europeana.eu/rights/rr-f/info:eu-repo/semantics/openAccess