106588 20210902121927.0 doi 10.1021/acs.nanolett.0c02163 sideral 120307 ART-2020-120307 eng (orcid)0000-0001-7625-4806 Piñol, R. Universidad de Zaragoza Real-Time Intracellular Temperature Imaging Using Lanthanide-Bearing Polymeric Micelles 2020 Access copy available to the general public Unrestricted Measurement of thermogenesis in individual cells is a remarkable challenge due to the complexity of the biochemical environment (such as pH and ionic strength) and to the rapid and yet not well-understood heat transfer mechanisms throughout the cell. Here, we present a unique system for intracellular temperature mapping in a fluorescence microscope (uncertainty of 0.2 K) using rationally designed luminescent Ln3+-bearing polymeric micellar probes (Ln = Sm, Eu) incubated in breast cancer MDA-MB468 cells. Two-dimensional (2D) thermal images recorded increasing the temperature of the cells culture medium between 296 and 304 K shows inhomogeneous intracellular temperature progressions up to ~20 degrees and subcellular gradients of ~5 degrees between the nucleolus and the rest of the cell, illustrating the thermogenic activity of the different organelles and highlighting the potential of this tool to study intracellular processes. info:eu-repo/grantAgreement/ES/DGA/E11-17R info:eu-repo/grantAgreement/EC/H2020/801305/EU/Nanoparticles-based 2D thermal bioimaging technologies/NanoTBTech This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 801305-NanoTBTech info:eu-repo/grantAgreement/EC/H2020/829162/EU/Redesigning biocatalysis: Thermal-tuning of one-pot multienzymatic cascades by nanoactuation/HOTZYMES This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 829162-HOTZYMES info:eu-repo/grantAgreement/ES/MINECO-FEDER/PGC2018-095795-B-I00 info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 11.189 2020 CHEMISTRY, PHYSICAL 22 / 162 = 0.136 2020 Q1 T1 MATERIALS SCIENCE, MULTIDISCIPLINARY 32 / 333 = 0.096 2020 Q1 T1 NANOSCIENCE & NANOTECHNOLOGY 15 / 106 = 0.142 2020 Q1 T1 PHYSICS, CONDENSED MATTER 11 / 69 = 0.159 2020 Q1 T1 CHEMISTRY, MULTIDISCIPLINARY 20 / 178 = 0.112 2020 Q1 T1 PHYSICS, APPLIED 15 / 160 = 0.094 2020 Q1 T1 4.852 2020 Bioengineering 2020 Q1 Chemistry (miscellaneous) 2020 Q1 Nanoscience and Nanotechnology 2020 Q1 Materials Science (miscellaneous) 2020 Q1 Mechanical Engineering 2020 Q1 Condensed Matter Physics 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Zeler, J. Brites, C.D.S. Gu, Y. (orcid)0000-0002-1819-4785 Téllez, P. Universidad de Zaragoza Carneiro Neto, A.N. da Silva, T.E. (orcid)0000-0002-6600-1618 Moreno-Loshuertos, R. Universidad de Zaragoza (orcid)0000-0001-8971-7355 Fernandez-Silva, P. Universidad de Zaragoza Gallego, A.I. (orcid)0000-0003-3043-147X Martinez-Lostao, L. Universidad de Zaragoza (orcid)0000-0002-8797-0813 Martínez, A. Universidad de Zaragoza Carlos, L.D. (orcid)0000-0003-0828-3212 Millán, A. Universidad de Zaragoza 5008 785 Universidad de Zaragoza Dpto. Ingeniería Electrón.Com. Área Tecnología Electrónica 1011 566 Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Inmunología 0 X Universidad de Zaragoza Serv.Gral. Apoyo Investigación División: Serv. Transversales 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 2003 395 Universidad de Zaragoza Dpto. Física Materia Condensa. Área Física Materia Condensada 20, 9 (2020), 6466-6472 Nano lett. Nano Letters 1530-6984 3119560 http://zaguan.unizar.es/record/106588/files/texto_completo.pdf Postprint 1814284 http://zaguan.unizar.es/record/106588/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:106588 articulos driver 2021-09-02-10:52:30 ARTICLE