000106625 001__ 106625
000106625 005__ 20220426091140.0
000106625 0247_ $$2doi$$a10.1016/j.anireprosci.2020.106567
000106625 0248_ $$2sideral$$a119870
000106625 037__ $$aART-2020-119870
000106625 041__ $$aeng
000106625 100__ $$0(orcid)0000-0002-1685-6845$$aGimeno-Martos, S.
000106625 245__ $$aUnderlying molecular mechanism in the modulation of the ram sperm acrosome reaction by progesterone and 17ß-estradiol
000106625 260__ $$c2020
000106625 5060_ $$aAccess copy available to the general public$$fUnrestricted
000106625 5203_ $$aSteroid hormones progesterone (P4) and 17ß-estradiol (E2) not only have important functions in regulation of reproductive processes in mammals but also have direct effects on spermatozoa. There can be induction of the acrosome reaction in ram spermatozoa by P4 and E2 and, in the present study, there was further investigation of mechanisms underlying this effect. In a medium containing agents that increase cAMP, the presence of both P4 and E2 led to changes in the localization of proteins phosphorylated in tyrosine residues evaluated by indirect immunofluorescence. The inclusion of P4 at 1 µM in the media induced an increase in Ca2+i and mobilization in the area of the acrosome (Fluo-4 and Rhod-5 staining, respectively), an increase in ROS (H2DCFDA staining) and a substantial disruption of the acrosome (evaluated using RCA), while E2 did not have these effects. There were no effects on cAMP concentrations or PKA activity with inclusion of these hormones in the media. The inclusion of P4 at 100 pM in the media led to changes in values for sperm kinematic variables which could indicate there was an inhibition of the hyperactivation caused by agents that induce an increase in cAMP concentrations. In conclusion, results from the present study indicate that P4 and E2 promote mechanisms regulating the acrosome reaction in ram spermatozoa, however, these effects on mechanisms are different for the two hormones, and for E2, require further clarification.
000106625 536__ $$9info:eu-repo/grantAgreement/ES/CICYT-FEDER/AGL2014-57863-R$$9info:eu-repo/grantAgreement/ES/DGA/A07-17R$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2017-83799-R$$9info:eu-repo/grantAgreement/ES/MINECO/BES-2015-072034$$9info:eu-repo/grantAgreement/ES/PIVEV/AGL-2016-81890-REDT
000106625 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000106625 590__ $$a2.145$$b2020
000106625 591__ $$aAGRICULTURE, DAIRY & ANIMAL SCIENCE$$b23 / 63 = 0.365$$c2020$$dQ2$$eT2
000106625 591__ $$aVETERINARY SCIENCES$$b43 / 146 = 0.295$$c2020$$dQ2$$eT1
000106625 591__ $$aREPRODUCTIVE BIOLOGY$$b26 / 30 = 0.867$$c2020$$dQ4$$eT3
000106625 592__ $$a0.572$$b2020
000106625 593__ $$aAnimal Science and Zoology$$c2020$$dQ2
000106625 593__ $$aMedicine (miscellaneous)$$c2020$$dQ2
000106625 593__ $$aFood Animals$$c2020$$dQ2
000106625 593__ $$aEndocrinology$$c2020$$dQ2
000106625 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000106625 700__ $$0(orcid)0000-0001-6733-9373$$aMiguel-Jiménez, S.$$uUniversidad de Zaragoza
000106625 700__ $$0(orcid)0000-0003-1997-4262$$aCasao, A.$$uUniversidad de Zaragoza
000106625 700__ $$0(orcid)0000-0001-9842-6197$$aCebrián-Pérez, J.A.$$uUniversidad de Zaragoza
000106625 700__ $$0(orcid)0000-0003-1306-8517$$aMuiño-Blanco, T.$$uUniversidad de Zaragoza
000106625 700__ $$0(orcid)0000-0002-2312-6402$$aPérez-Pe, R.$$uUniversidad de Zaragoza
000106625 7102_ $$11002$$2819$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Zoología
000106625 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000106625 773__ $$g221, 106567 (2020), [12 pp]$$pAnim. reprod. sci.$$tAnimal Reproduction Science$$x0378-4320
000106625 8564_ $$s700082$$uhttps://zaguan.unizar.es/record/106625/files/texto_completo.pdf$$yPostprint
000106625 8564_ $$s776444$$uhttps://zaguan.unizar.es/record/106625/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000106625 909CO $$ooai:zaguan.unizar.es:106625$$particulos$$pdriver
000106625 951__ $$a2022-04-26-08:56:29
000106625 980__ $$aARTICLE