106645 20230519145402.0 doi 10.3390/toxins13030201 sideral 124617 ART-2021-124617 eng Bervis, Noemi (orcid)0000-0002-9798-1268 Field monitoring of aflatoxins in feed and milk of high-yielding dairy cows under two feeding systems 2021 Access copy available to the general public Unrestricted Aflatoxin M1 (AFM1) is a hydroxylated metabolite of aflatoxin B1 (AFB1) that can be excreted in milk of cows after consuming contaminated feed. The aim of this study consisted of a field monitoring to assess the contamination levels of AFB1 in 60 feed samples from two feeding systems for high-yielding dairy cows and of AFM1 in the corresponding raw milk samples. The aflatoxins were analyzed by in-house validated methods based on high-performance liquid chromatography (HPLC) with fluorescence detection. AFB1 was detected in 55% of feed samples (mean 0.61 μg/kg, with 2 samples exceeding the European Union (EU) maximum level set at 5 μg/kg), with greater incidence and concentration in compound feed than in unifeed rations (p < 0.05). AFM1 was detected in 38.3% milk samples (mean 12.6 ng/kg, with 5 samples exceeding the EU maximum level set at 50 ng/kg), with a higher occurrence in milk of cows fed compound feed, as well as in spring milk compared to that produced in winter. The overall transfer ratio of aflatoxins from feed to milk was 3.22%, being higher in cows fed with compound feed and in spring milkings. In a selection of positive matched samples (n = 22), the ratio AFM1/AFB1 exceeded the European Food Safety Authority (EFSA) estimated 6% threshold for high-yielding dairy cow. info:eu-repo/grantAgreement/ES/DGA/A06-20R info:eu-repo/grantAgreement/ES/DGA-FEDER/2014-2020 info:eu-repo/grantAgreement/ES/MINECO/AGL2014-57069-R info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 5.075 2021 TOXICOLOGY 20 / 94 = 0.213 2021 Q1 T1 FOOD SCIENCE & TECHNOLOGY 40 / 144 = 0.278 2021 Q2 T1 0.884 2021 Toxicology 2021 Q1 Health, Toxicology and Mutagenesis 2021 Q1 6.6 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Lorán, Susana Universidad de Zaragoza (orcid)0000-0002-1961-8551 Juan, Teresa Universidad de Zaragoza (orcid)0000-0002-4985-298X Carramiñana, Juan José Universidad de Zaragoza (orcid)0000-0002-3320-9295 Herrera, Antonio Universidad de Zaragoza (orcid)0000-0001-7195-3640 Ariño, Agustín Universidad de Zaragoza (orcid)0000-0001-6325-7100 Herrera, Marta Universidad de Zaragoza (orcid)0000-0002-2469-0363 2008 640 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Nutrición Bromatología 13, 3 (2021), [15 pp.] Toxins Toxins 2072-6651 377442 http://zaguan.unizar.es/record/106645/files/texto_completo.pdf Versión publicada 2620831 http://zaguan.unizar.es/record/106645/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:106645 articulos driver 2023-05-18-13:41:51 ARTICLE