106657 20230519145411.0 doi 10.3390/plants10081527 sideral 124653 ART-2021-124653 eng Langa Lomba, Natalia Activity of anthracenediones and flavoring phenols in hydromethanolic extracts of Rubia tinctorum against grapevine phytopathogenic fungi 2021 Access copy available to the general public Unrestricted In this work, the chemical composition of Rubia tinctorum root hydromethanolic extract was analyzed by GC–MS, and over 50 constituents were identified. The main phytochemicals were alizarin-related anthraquinones and flavoring phenol compounds. The antifungal activity of this extract, alone and in combination with chitosan oligomers (COS) or with stevioside, was evaluated against the pathogenic taxa Diplodia seriata, Dothiorella viticola and Neofusicoccum parvum, responsible for the so-called Botryosphaeria dieback of grapevine. In vitro mycelial growth inhibition tests showed remarkable activity for the pure extract, with EC50 and EC90 values as low as 66 and 88 μg·mL−1, respectively. Nonetheless, enhanced activity was attained upon the formation of conjugate complexes with COS or with stevioside, with synergy factors of up to 5.4 and 3.3, respectively, resulting in EC50 and EC90 values as low as 22 and 56 μg·mL−1, respectively. The conjugate with the best performance (COS–R. tinctorum extract) was then assayed ex situ on autoclaved grapevine wood against D. seriata, confirming its antifungal behavior on this plant material. Finally, the same conjugate was evaluated in greenhouse assays on grafted grapevine plants artificially inoculated with the three aforementioned fungal species, resulting in a significant reduction in the infection rate in all cases. This natural antifungal compound represents a promising alternative for developing sustainable control methods against grapevine trunk diseases info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.658 2021 PLANT SCIENCES 39 / 240 = 0.162 2021 Q1 T1 0.765 2021 Plant Science 2021 Q1 Ecology, Evolution, Behavior and Systematics 2021 Q1 3.6 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Sánchez Hernández, Eva Buzón Durán, Laura González García, Vicente Casanova Gascón, José Universidad de Zaragoza (orcid)0000-0003-3009-0935 Martín Gil, Jesús Martín Ramos, Pablo Universidad de Zaragoza (orcid)0000-0003-2713-2786 5011 500 Universidad de Zaragoza Dpto. CC.Agrar.y Medio Natural Area Ingeniería Agroforestal 5011 705 Universidad de Zaragoza Dpto. CC.Agrar.y Medio Natural Área Producción Vegetal 10, 8 (2021), 1527 [17 pp.] Plants 2223-7747 1241338 http://zaguan.unizar.es/record/106657/files/texto_completo.pdf Versión publicada 2506656 http://zaguan.unizar.es/record/106657/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:106657 articulos driver 2023-05-18-13:55:17 ARTICLE