000106684 001__ 106684
000106684 005__ 20240104111818.0
000106684 0247_ $$2doi$$a10.3390/ijms22136822
000106684 0248_ $$2sideral$$a124701
000106684 037__ $$aART-2021-124701
000106684 041__ $$aeng
000106684 100__ $$0(orcid)0000-0003-2454-2114$$aLópez-Pérez, Óscar
000106684 245__ $$aCerebrospinal fluid and plasma small extracellular vesicles and mirnas as biomarkers for prion diseases
000106684 260__ $$c2021
000106684 5060_ $$aAccess copy available to the general public$$fUnrestricted
000106684 5203_ $$aDiagnosis of transmissible spongiform encephalopathies (TSEs), or prion diseases, is based on the detection of proteinase K (PK)-resistant PrPSc in post-mortem tissues as indication of infection and disease. Since PrPSc detection is not considered a reliable method for in vivo diagnosis in most TSEs, it is of crucial importance to identify an alternative source of biomarkers to provide useful alternatives for current diagnostic methodology. Ovine scrapie is the prototype of TSEs and has been known for a long time. Using this natural model of TSE, we investigated the presence of PrPSc in exosomes derived from plasma and cerebrospinal fluid (CSF) by protein misfolding cyclic amplification (PMCA) and the levels of candidate microRNAs (miRNAs) by quantitative PCR (qPCR). Significant scrapie-associated increase was found for miR-21-5p in plasma-derived but not in CSF-derived exosomes. However, miR-342-3p, miR-146a-5p, miR-128-3p and miR-21-5p displayed higher levels in total CSF from scrapie-infected sheep. The analysis of overexpressed miRNAs in this biofluid, together with plasma exosomal miR-21-5p, could help in scrapie diagnosis once the presence of the disease is suspected. In addition, we found the presence of PrPSc in most CSF-derived exosomes from clinically affected sheep, which may facilitate in vivo diagnosis of prion diseases, at least during the clinical stage.
000106684 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A19-17R$$9info:eu-repo/grantAgreement/ES/DGA-FEDER/Construyendo Europa desde Aragón$$9info:eu-repo/grantAgreement/EUR/INTERREG-POCTEFA/EFA-148-16 REDPRION$$9info:eu-repo/grantAgreement/EUR/INTERREG-V-POCTEFA-2014-2020$$9info:eu-repo/grantAgreement/ES/MICINN/RTI2018-098711-B-I00$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2015-67945-P
000106684 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000106684 590__ $$a6.208$$b2021
000106684 592__ $$a1.176$$b2021
000106684 594__ $$a6.9$$b2021
000106684 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b69 / 297 = 0.232$$c2021$$dQ1$$eT1
000106684 593__ $$aComputer Science Applications$$c2021$$dQ1
000106684 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b50 / 179 = 0.279$$c2021$$dQ2$$eT1
000106684 593__ $$aInorganic Chemistry$$c2021$$dQ1
000106684 593__ $$aSpectroscopy$$c2021$$dQ1
000106684 593__ $$aOrganic Chemistry$$c2021$$dQ1
000106684 593__ $$aPhysical and Theoretical Chemistry$$c2021$$dQ1
000106684 593__ $$aMolecular Biology$$c2021$$dQ1
000106684 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000106684 700__ $$0(orcid)0000-0002-5228-248X$$aSanz-Rubio, David
000106684 700__ $$0(orcid)0000-0002-6806-9990$$aHernaiz, Adelaida$$uUniversidad de Zaragoza
000106684 700__ $$0(orcid)0000-0002-0388-9429$$aBetancor, Marina$$uUniversidad de Zaragoza
000106684 700__ $$0(orcid)0000-0001-9075-2764$$aOtero, Alicia$$uUniversidad de Zaragoza
000106684 700__ $$aCastilla, Joaquín
000106684 700__ $$aAndréoletti, Olivier
000106684 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, Juan José$$uUniversidad de Zaragoza
000106684 700__ $$0(orcid)0000-0001-5740-0185$$aZaragoza, Pilar$$uUniversidad de Zaragoza
000106684 700__ $$0(orcid)0000-0002-2746-3932$$aBolea, Rosa$$uUniversidad de Zaragoza
000106684 700__ $$0(orcid)0000-0002-7243-1737$$aToivonen, Janne$$uUniversidad de Zaragoza
000106684 700__ $$0(orcid)0000-0001-6016-4726$$aMartín-Burriel, Inmaculada$$uUniversidad de Zaragoza
000106684 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000106684 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000106684 773__ $$g22, 13 (2021), [16 pp.]$$pInt. j. mol. sci.$$tInternational Journal of Molecular Sciences$$x1661-6596
000106684 8564_ $$s1661182$$uhttps://zaguan.unizar.es/record/106684/files/texto_completo.pdf$$yVersión publicada
000106684 8564_ $$s2734959$$uhttps://zaguan.unizar.es/record/106684/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
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000106684 951__ $$a2024-01-04-11:06:38
000106684 980__ $$aARTICLE