106684 20240104111818.0 doi 10.3390/ijms22136822 sideral 124701 ART-2021-124701 eng López-Pérez, Óscar (orcid)0000-0003-2454-2114 Cerebrospinal fluid and plasma small extracellular vesicles and mirnas as biomarkers for prion diseases 2021 Access copy available to the general public Unrestricted Diagnosis of transmissible spongiform encephalopathies (TSEs), or prion diseases, is based on the detection of proteinase K (PK)-resistant PrPSc in post-mortem tissues as indication of infection and disease. Since PrPSc detection is not considered a reliable method for in vivo diagnosis in most TSEs, it is of crucial importance to identify an alternative source of biomarkers to provide useful alternatives for current diagnostic methodology. Ovine scrapie is the prototype of TSEs and has been known for a long time. Using this natural model of TSE, we investigated the presence of PrPSc in exosomes derived from plasma and cerebrospinal fluid (CSF) by protein misfolding cyclic amplification (PMCA) and the levels of candidate microRNAs (miRNAs) by quantitative PCR (qPCR). Significant scrapie-associated increase was found for miR-21-5p in plasma-derived but not in CSF-derived exosomes. However, miR-342-3p, miR-146a-5p, miR-128-3p and miR-21-5p displayed higher levels in total CSF from scrapie-infected sheep. The analysis of overexpressed miRNAs in this biofluid, together with plasma exosomal miR-21-5p, could help in scrapie diagnosis once the presence of the disease is suspected. In addition, we found the presence of PrPSc in most CSF-derived exosomes from clinically affected sheep, which may facilitate in vivo diagnosis of prion diseases, at least during the clinical stage. info:eu-repo/grantAgreement/ES/DGA/A19-17R info:eu-repo/grantAgreement/ES/DGA-FEDER/Construyendo Europa desde Aragón info:eu-repo/grantAgreement/EUR/INTERREG-POCTEFA/EFA-148-16 REDPRION info:eu-repo/grantAgreement/EUR/INTERREG-V-POCTEFA-2014-2020 info:eu-repo/grantAgreement/ES/MICINN/RTI2018-098711-B-I00 info:eu-repo/grantAgreement/ES/MINECO/AGL2015-67945-P info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 6.208 2021 BIOCHEMISTRY & MOLECULAR BIOLOGY 69 / 297 = 0.232 2021 Q1 T1 CHEMISTRY, MULTIDISCIPLINARY 50 / 179 = 0.279 2021 Q2 T1 1.176 2021 Computer Science Applications 2021 Q1 Inorganic Chemistry 2021 Q1 Spectroscopy 2021 Q1 Organic Chemistry 2021 Q1 Physical and Theoretical Chemistry 2021 Q1 Molecular Biology 2021 Q1 6.9 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Sanz-Rubio, David (orcid)0000-0002-5228-248X Hernaiz, Adelaida Universidad de Zaragoza (orcid)0000-0002-6806-9990 Betancor, Marina Universidad de Zaragoza (orcid)0000-0002-0388-9429 Otero, Alicia Universidad de Zaragoza (orcid)0000-0001-9075-2764 Castilla, Joaquín Andréoletti, Olivier Badiola, Juan José Universidad de Zaragoza (orcid)0000-0002-7173-7216 Zaragoza, Pilar Universidad de Zaragoza (orcid)0000-0001-5740-0185 Bolea, Rosa Universidad de Zaragoza (orcid)0000-0002-2746-3932 Toivonen, Janne Universidad de Zaragoza (orcid)0000-0002-7243-1737 Martín-Burriel, Inmaculada Universidad de Zaragoza (orcid)0000-0001-6016-4726 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 22, 13 (2021), [16 pp.] Int. j. mol. sci. International Journal of Molecular Sciences 1661-6596 1661182 http://zaguan.unizar.es/record/106684/files/texto_completo.pdf Versión publicada 2734959 http://zaguan.unizar.es/record/106684/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:106684 articulos driver 2024-01-04-11:06:38 ARTICLE