Thioredoxin dependent changes in the redox states of FurA from Anabaena sp. PCC7120

Guío, Jorge (Universidad de Zaragoza) ; Bes, Mª Teresa (Universidad de Zaragoza) ; Balsera, Mónica ; Calvo-Beguería, L. ; Sevilla, E. (Universidad de Zaragoza) ; Peleato, Mª Luisa (Universidad de Zaragoza) ; Fillat, Mª Francisca (Universidad de Zaragoza)

Resumen: FurA is a multifunctional regulator in cyanobacteria that contains five cysteines, four of them arranged into two CXXC motifs. Lack of a structural zinc ion enables FurA to develop disulfide reductase activity. In vivo, FurA displays several redox isoforms, and the oxidation state of its cysteines determines its activity as regulator and its ability to bind different metabolites. Because of the relationship between FurA and the control of genes involved in oxidative stress defense and photosynthetic metabolism, we sought to investigate the role of type m thioredoxin TrxA as a potential redox partner mediating dithiol-disulfide exchange reactions necessary to facilitate the interaction of FurA with its different ligands. Both in vitro cross-linking assays and in vivo two-hybrid studies confirmed the interaction between FurA and TrxA. Light to dark transitions resulted in reversible oxidation of a fraction of the regulator present in Anabaena sp. PCC7120. Reconstitution of an electron transport chain using E. coli NADPH-thioredoxin-reductase followed by alkylation of FurA reduced cysteines evidenced the ability of TrxA to reduce FurA. Furthermore, the use of site-directed mutants allowed us to propose a plausible mechanism for FurA reduction. These results point to TrxA as one of the redox partners that modulates FurA performance.

Idioma: Inglés

DOI: 10.3390/antiox10060913

Año: 2021

Publicado en: Antioxidants 10, 6 (2021), 913 [14 pp.]
ISSN: 2076-3921

Factor impacto JCR: 7.675 (2021)

Categ. JCR: BIOCHEMISTRY & MOLECULAR BIOLOGY rank: 50 / 297 = 0.168 (2021) - Q1 - T1
Categ. JCR: FOOD SCIENCE & TECHNOLOGY rank: 12 / 144 = 0.083 (2021) - Q1 - T1
Categ. JCR: CHEMISTRY, MEDICINAL rank: 4 / 63 = 0.063 (2021) - Q1 - T1

Factor impacto CITESCORE: 6.5 - Biochemistry, Genetics and Molecular Biology (Q2)

Factor impacto SCIMAGO: 1.008 - Biochemistry (Q1) - Physiology (Q1) - Molecular Biology (Q1) - Clinical Biochemistry (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA-FEDER/E35-17R
Financiación: info:eu-repo/grantAgreement/ES/MCIU/PID2019-104889GB-I00
Financiación: info:eu-repo/grantAgreement/ES/MINECO/BFU2016-77671-P

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Fisiología Vegetal (Dpto. Bioq.Biolog.Mol. Celular)
Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)