106688 20230519145447.0 doi 10.3390/antiox10060913 sideral 124624 ART-2021-124624 eng Guío, Jorge Universidad de Zaragoza (orcid)0000-0002-0474-255X Thioredoxin dependent changes in the redox states of FurA from Anabaena sp. PCC7120 2021 Access copy available to the general public Unrestricted FurA is a multifunctional regulator in cyanobacteria that contains five cysteines, four of them arranged into two CXXC motifs. Lack of a structural zinc ion enables FurA to develop disulfide reductase activity. In vivo, FurA displays several redox isoforms, and the oxidation state of its cysteines determines its activity as regulator and its ability to bind different metabolites. Because of the relationship between FurA and the control of genes involved in oxidative stress defense and photosynthetic metabolism, we sought to investigate the role of type m thioredoxin TrxA as a potential redox partner mediating dithiol-disulfide exchange reactions necessary to facilitate the interaction of FurA with its different ligands. Both in vitro cross-linking assays and in vivo two-hybrid studies confirmed the interaction between FurA and TrxA. Light to dark transitions resulted in reversible oxidation of a fraction of the regulator present in Anabaena sp. PCC7120. Reconstitution of an electron transport chain using E. coli NADPH-thioredoxin-reductase followed by alkylation of FurA reduced cysteines evidenced the ability of TrxA to reduce FurA. Furthermore, the use of site-directed mutants allowed us to propose a plausible mechanism for FurA reduction. These results point to TrxA as one of the redox partners that modulates FurA performance. info:eu-repo/grantAgreement/ES/DGA-FEDER/E35-17R info:eu-repo/grantAgreement/ES/MCIU/PID2019-104889GB-I00 info:eu-repo/grantAgreement/ES/MINECO/BFU2016-77671-P info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 7.675 2021 BIOCHEMISTRY & MOLECULAR BIOLOGY 50 / 297 = 0.168 2021 Q1 T1 FOOD SCIENCE & TECHNOLOGY 12 / 144 = 0.083 2021 Q1 T1 CHEMISTRY, MEDICINAL 4 / 63 = 0.063 2021 Q1 T1 1.008 2021 Biochemistry 2021 Q1 Physiology 2021 Q1 Molecular Biology 2021 Q1 Clinical Biochemistry 2021 Q1 6.5 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Bes, Mª Teresa Universidad de Zaragoza (orcid)0000-0002-8181-2689 Balsera, Mónica Calvo-Beguería, L. (orcid)0000-0001-5327-4046 Sevilla, E. Universidad de Zaragoza (orcid)0000-0001-6435-3540 Peleato, Mª Luisa Universidad de Zaragoza (orcid)0000-0002-2742-3711 Fillat, Mª Francisca Universidad de Zaragoza (orcid)0000-0001-8644-4574 1002 412 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Fisiología Vegetal 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 10, 6 (2021), 913 [14 pp.] Antioxidants Antioxidants 2076-3921 1856832 http://zaguan.unizar.es/record/106688/files/texto_completo.pdf Versión publicada 2707540 http://zaguan.unizar.es/record/106688/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:106688 articulos driver 2023-05-18-14:39:37 ARTICLE