106753 20231221135831.0 doi 10.3390/foods10081897 sideral 124710 ART-2021-124710 eng Serrano, M.J. (orcid)0000-0003-3850-9833 Optimization and validation of a new microbial inhibition test for the detection of antimicrobial residues in living animals intended for human consumption 2021 Access copy available to the general public Unrestricted Even though antibiotics are necessary in livestock production, they can be harmful not only due to their toxicity, but also in view of their contribution to the emergence of antimicrobial resistance. Screening tests based on microbial growth inhibition appeared to be useful tools to prevent its entry into the food chain. They have nevertheless been traditionally carried out post mortem, leading to great economical loss and harm to the environment in case a positive sample is found. Hence, the objective was to evaluate the use of a screening test as an ante mortem alternative for the detection of antibiotic residues in meat: thus, Explorer®-Blood test was optimized and validated. After adapting the procedure for matrix preparation, the assay parameters were assessed from 344 antibiotic-free blood serum samples. Limits of Detection (LoDs) were defined by spiking blood serum with several of the most common antimicrobials used in veterinary practice. LoDs were similar to those obtained for meat and were at or below the maximum residue limits set by EU legislation for muscle. Analyses of in vivo injected samples, previously characterized by LC-MS/MS, demonstrated the method’s accuracy and proved that Explorer®-Blood can be considered a suitable alternative to conventional post mortem screening methods. info:eu-repo/grantAgreement/EUR/INTERREG-V-A-POCTEFA/TESTACOS-EFA152/16 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 5.561 2021 FOOD SCIENCE & TECHNOLOGY 35 / 144 = 0.243 2021 Q1 T1 0.726 2021 Food Science 2021 Q1 Plant Science 2021 Q1 Microbiology 2021 Q1 Health Professions (miscellaneous) 2021 Q1 4.1 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Mata, L. García-Gonzalo, D. Universidad de Zaragoza (orcid)0000-0002-7629-8101 Antón, A. Razquin, P. Condón, S. Universidad de Zaragoza (orcid)0000-0002-1902-0648 Pagán, R. Universidad de Zaragoza (orcid)0000-0002-0238-6328 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 10, 8 (2021), 1897 Foods Foods 2304-8158 1653762 http://zaguan.unizar.es/record/106753/files/texto_completo.pdf Versión publicada 2771349 http://zaguan.unizar.es/record/106753/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:106753 articulos driver 2023-12-21-13:43:04 ARTICLE