White-tailed deer S96 prion protein does not support stable in vitro propagation of most common CWD strains
Resumen: PrPC variation at residue 96 (G/S) plays an important role in the epidemiology of chronic wasting disease (CWD) in exposed white-tailed deer populations. In vivo studies have demonstrated the protective effect of serine at codon 96, which hinders the propagation of common CWD strains when expressed in homozygosis and increases the survival period of S96/wt heterozygous deer after challenge with CWD. Previous in vitro studies of the transmission barrier suggested that following a single amplification step, wt and S96 PrPC were equally susceptible to misfolding when seeded with various CWD prions. When we performed serial prion amplification in vitro using S96-PrPC, we observed a reduction in the efficiency of propagation with the Wisc-1 or CWD2 strains, suggesting these strains cannot stably template their conformations on this PrPC once the primary sequence has changed after the first round of replication. Our data shows the S96-PrPC polymorphism is detrimental to prion conversion of some CWD strains. These data suggests that deer homozygous for S96-PrPC may not sustain prion transmission as compared to a deer expressing G96-PrPC.
Idioma: Inglés
DOI: 10.1038/s41598-021-90606-8
Año: 2021
Publicado en: Scientific reports (Nature Publishing Group) 11 (2021), 11193 [10 pp.]
ISSN: 2045-2322

Factor impacto JCR: 4.997 (2021)
Categ. JCR: MULTIDISCIPLINARY SCIENCES rank: 19 / 74 = 0.257 (2021) - Q2 - T1
Factor impacto SCIMAGO: 1.005 - Multidisciplinary (Q1)

Tipo y forma: Artículo (Versión definitiva)

Creative Commons Debe reconocer adecuadamente la autoría, proporcionar un enlace a la licencia e indicar si se han realizado cambios. Puede hacerlo de cualquier manera razonable, pero no de una manera que sugiera que tiene el apoyo del licenciador o lo recibe por el uso que hace.


Exportado de SIDERAL (2023-05-18-15:12:27)


Visitas y descargas

Este artículo se encuentra en las siguientes colecciones:
Artículos



 Registro creado el 2021-09-01, última modificación el 2023-05-19


Versión publicada:
 PDF
Valore este documento:

Rate this document:
1
2
3
 
(Sin ninguna reseña)