000107318 001__ 107318
000107318 005__ 20210913154735.0
000107318 0247_ $$2doi$$a10.1002/elps.201500577
000107318 0248_ $$2sideral$$a94984
000107318 037__ $$aART-2016-94984
000107318 041__ $$aeng
000107318 100__ $$0(orcid)0000-0002-8460-6020$$aJimenez, M.S.$$uUniversidad de Zaragoza
000107318 245__ $$aEvaluation of agarose gel electrophoresis for characterization of silver nanoparticles in industrial products
000107318 260__ $$c2016
000107318 5060_ $$aAccess copy available to the general public$$fUnrestricted
000107318 5203_ $$aAgarose gel electrophoresis (AGE) has been used extensively for characterization of pure nanomaterials or mixtures of pure nanomaterials. We have evaluated the use of AGE for characterization of Ag nanoparticles (NPs) in an industrial product (described as strong antiseptic). Influence of different stabilizing agents (PEG, SDS, and sodium dodecylbenzenesulfonate), buffers (TBE and Tris Glycine), and functionalizing agents (mercaptosuccinic acid (TMA) and proteins) has been investigated for the characterization of AgNPs in the industrial product using different sizes-AgNPs standards. The use of 1% SDS, 0.1% TMA, and Tris Glycine in gel, electrophoresis buffer and loading buffer led to the different sizes-AgNPs standards moved according to their size/charge ratio (obtaining a linear relationship between apparent mobility and mean diameter). After using SDS and TMA, the behavior of the AgNPs in the industrial product (containing a casein matrix) was completely different, being not possible their size characterization. However we demonstrated that AGE with LA-ICP-MS detection is an alternative method to confirm the protein corona formation between the industrial product and two proteins (BSA and transferrin) maintaining NPs-protein binding (what is not possible using SDS-PAGE).
000107318 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2012-38091-C02-00
000107318 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000107318 590__ $$a2.744$$b2016
000107318 591__ $$aCHEMISTRY, ANALYTICAL$$b24 / 76 = 0.316$$c2016$$dQ2$$eT1
000107318 591__ $$aBIOCHEMICAL RESEARCH METHODS$$b32 / 77 = 0.416$$c2016$$dQ2$$eT2
000107318 592__ $$a0.847$$b2016
000107318 593__ $$aAnalytical Chemistry$$c2016$$dQ1
000107318 593__ $$aClinical Biochemistry$$c2016$$dQ2
000107318 593__ $$aBiochemistry$$c2016$$dQ2
000107318 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000107318 700__ $$0(orcid)0000-0001-5002-7197$$aLuque-Alled, J.
000107318 700__ $$0(orcid)0000-0003-1400-9041$$aGomez, T.$$uUniversidad de Zaragoza
000107318 700__ $$0(orcid)0000-0002-4070-8607$$aCastillo, J.R.$$uUniversidad de Zaragoza
000107318 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000107318 773__ $$g37, 10 (2016), 1376-1383$$pElectrophoresis$$tELECTROPHORESIS$$x0173-0835
000107318 8564_ $$s1033504$$uhttps://zaguan.unizar.es/record/107318/files/texto_completo.pdf$$yPostprint
000107318 8564_ $$s849244$$uhttps://zaguan.unizar.es/record/107318/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000107318 909CO $$ooai:zaguan.unizar.es:107318$$particulos$$pdriver
000107318 951__ $$a2021-09-13-13:16:13
000107318 980__ $$aARTICLE