000107419 001__ 107419
000107419 005__ 20230519145505.0
000107419 0247_ $$2doi$$a10.1038/s41598-021-96818-2
000107419 0248_ $$2sideral$$a124754
000107419 037__ $$aART-2021-124754
000107419 041__ $$aeng
000107419 100__ $$0(orcid)0000-0002-1590-3347$$aMarín, Belén$$uUniversidad de Zaragoza
000107419 245__ $$aClassical BSE prions emerge from asymptomatic pigs challenged with atypical/Nor98 scrapie
000107419 260__ $$c2021
000107419 5060_ $$aAccess copy available to the general public$$fUnrestricted
000107419 5203_ $$aPigs are susceptible to infection with the classical bovine spongiform encephalopathy (C-BSE) agent following experimental inoculation, and PrPSc accumulation was detected in porcine tissues after the inoculation of certain scrapie and chronic wasting disease isolates. However, a robust transmission barrier has been described in this species and, although they were exposed to C-BSE agent in many European countries, no cases of natural transmissible spongiform encephalopathies (TSE) infections have been reported in pigs. Transmission of atypical scrapie to bovinized mice resulted in the emergence of C-BSE prions. Here, we conducted a study to determine if pigs are susceptible to atypical scrapie. To this end, 12, 8–9-month-old minipigs were intracerebrally inoculated with two atypical scrapie sources. Animals were euthanized between 22- and 72-months post inoculation without clinical signs of TSE. All pigs tested negative for PrPSc accumulation by enzyme immunoassay, immunohistochemistry, western blotting and bioassay in porcine PrP mice. Surprisingly, in vitro protein misfolding cyclic amplification demonstrated the presence of C-BSE prions in different brain areas from seven pigs inoculated with both atypical scrapie isolates. Our results suggest that pigs exposed to atypical scrapie prions could become a reservoir for C-BSE and corroborate that C-BSE prions emerge during interspecies passage of atypical scrapie.
000107419 536__ $$9info:eu-repo/grantAgreement/EUR/INTERREG-V-POCTEFA-2014-2020$$9info:eu-repo/grantAgreement/ES/MCIU/PID2019-105837RB-I00
000107419 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000107419 590__ $$a4.997$$b2021
000107419 592__ $$a1.005$$b2021
000107419 591__ $$aMULTIDISCIPLINARY SCIENCES$$b19 / 74 = 0.257$$c2021$$dQ2$$eT1
000107419 593__ $$aMultidisciplinary$$c2021$$dQ1
000107419 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000107419 700__ $$0(orcid)0000-0001-9075-2764$$aOtero, Alicia$$uUniversidad de Zaragoza
000107419 700__ $$aLugan, Séverine
000107419 700__ $$aEspinosa, Juan Carlos
000107419 700__ $$aMarín-Moreno, Alba
000107419 700__ $$aVidal, Enric
000107419 700__ $$0(orcid)0000-0002-0827-110X$$aHedman, Carlos
000107419 700__ $$0(orcid)0000-0001-7188-0461$$aRomero, Antonio$$uUniversidad de Zaragoza
000107419 700__ $$0(orcid)0000-0002-0935-7941$$aPumarola, Martí
000107419 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, Juan J.$$uUniversidad de Zaragoza
000107419 700__ $$aTorres, Juan María
000107419 700__ $$aAndréoletti, Oliver
000107419 700__ $$0(orcid)0000-0002-2746-3932$$aBolea, Rosa$$uUniversidad de Zaragoza
000107419 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000107419 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000107419 773__ $$g11 (2021), 17428 [8 pp.]$$pSci. rep. (Nat. Publ. Group)$$tScientific reports (Nature Publishing Group)$$x2045-2322
000107419 8564_ $$s963856$$uhttps://zaguan.unizar.es/record/107419/files/texto_completo.pdf$$yVersión publicada
000107419 8564_ $$s2533667$$uhttps://zaguan.unizar.es/record/107419/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000107419 909CO $$ooai:zaguan.unizar.es:107419$$particulos$$pdriver
000107419 951__ $$a2023-05-18-15:05:36
000107419 980__ $$aARTICLE