107419 20230519145505.0 doi 10.1038/s41598-021-96818-2 sideral 124754 ART-2021-124754 eng Marín, Belén Universidad de Zaragoza (orcid)0000-0002-1590-3347 Classical BSE prions emerge from asymptomatic pigs challenged with atypical/Nor98 scrapie 2021 Access copy available to the general public Unrestricted Pigs are susceptible to infection with the classical bovine spongiform encephalopathy (C-BSE) agent following experimental inoculation, and PrPSc accumulation was detected in porcine tissues after the inoculation of certain scrapie and chronic wasting disease isolates. However, a robust transmission barrier has been described in this species and, although they were exposed to C-BSE agent in many European countries, no cases of natural transmissible spongiform encephalopathies (TSE) infections have been reported in pigs. Transmission of atypical scrapie to bovinized mice resulted in the emergence of C-BSE prions. Here, we conducted a study to determine if pigs are susceptible to atypical scrapie. To this end, 12, 8–9-month-old minipigs were intracerebrally inoculated with two atypical scrapie sources. Animals were euthanized between 22- and 72-months post inoculation without clinical signs of TSE. All pigs tested negative for PrPSc accumulation by enzyme immunoassay, immunohistochemistry, western blotting and bioassay in porcine PrP mice. Surprisingly, in vitro protein misfolding cyclic amplification demonstrated the presence of C-BSE prions in different brain areas from seven pigs inoculated with both atypical scrapie isolates. Our results suggest that pigs exposed to atypical scrapie prions could become a reservoir for C-BSE and corroborate that C-BSE prions emerge during interspecies passage of atypical scrapie. info:eu-repo/grantAgreement/EUR/INTERREG-V-POCTEFA-2014-2020 info:eu-repo/grantAgreement/ES/MCIU/PID2019-105837RB-I00 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.997 2021 MULTIDISCIPLINARY SCIENCES 19 / 74 = 0.257 2021 Q2 T1 1.005 2021 Multidisciplinary 2021 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Otero, Alicia Universidad de Zaragoza (orcid)0000-0001-9075-2764 Lugan, Séverine Espinosa, Juan Carlos Marín-Moreno, Alba Vidal, Enric Hedman, Carlos (orcid)0000-0002-0827-110X Romero, Antonio Universidad de Zaragoza (orcid)0000-0001-7188-0461 Pumarola, Martí (orcid)0000-0002-0935-7941 Badiola, Juan J. Universidad de Zaragoza (orcid)0000-0002-7173-7216 Torres, Juan María Andréoletti, Oliver Bolea, Rosa Universidad de Zaragoza (orcid)0000-0002-2746-3932 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 11 (2021), 17428 [8 pp.] Sci. rep. (Nat. Publ. Group) Scientific reports (Nature Publishing Group) 2045-2322 963856 http://zaguan.unizar.es/record/107419/files/texto_completo.pdf Versión publicada 2533667 http://zaguan.unizar.es/record/107419/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:107419 articulos driver 2023-05-18-15:05:36 ARTICLE