000108297 001__ 108297
000108297 005__ 20211115135504.0
000108297 0247_ $$2doi$$a10.1021/acsami.0c17043
000108297 0248_ $$2sideral$$a121195
000108297 037__ $$aART-2020-121195
000108297 041__ $$aeng
000108297 100__ $$0(orcid)0000-0002-0595-5514$$aGarcia-Salinas, Sara$$uUniversidad de Zaragoza
000108297 245__ $$aAntimicrobial Wound Dressings against Fluorescent and Methicillin-Sensitive Intracellular Pathogenic Bacteria
000108297 260__ $$c2020
000108297 5060_ $$aAccess copy available to the general public$$fUnrestricted
000108297 5203_ $$aThere is limited evidence indicating that drug-eluting dressings are clinically more effective than simple conventional dressings. To shed light on this concern, we have performed evidence-based research to evaluate the antimicrobial action of thymol (THY)-loaded antimicrobial dressings having antibiofilm forming ability, able to eradicate intracellular and extracellular pathogenic bacteria. We have used four different Staphylococcus aureus strains, including the ATCC 25923 strain, the Newman strain (methicillin-sensitive strain, MSSA) expressing the coral green fluorescent protein from the vector pCN47, and two clinical reference strains, Newman-(MSSA) and USA300-(methicillin-resistant strain), as traceable models of pathogenic bacteria commonly infecting skin and soft tissues. Compared to non-loaded dressings, THY-loaded polycaprolactone-based electrospun dressings were also able to eliminate pathogenic bacteria in coculture models based on infected murine macrophages. In addition, by using confocal microscopy and the conventional microdilution plating method, we corroborated the successful ability of THY in preventing also biofilm formation. Herein, we demonstrated that the use of wound dressings loaded with the natural monoterpenoid phenol derivative THY are able to eliminate biofilm formation and intracellular methicillin-sensitive S aureus more efficiently than with their corresponding THY-free counterparts.
000108297 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII-FEDER/CIBER-BBN$$9info:eu-repo/grantAgreement/ES/ISCIII-IIS/MS19-00092$$9info:eu-repo/grantAgreement/ES/MCIU/BES-2015-073735$$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2017-84473-R
000108297 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000108297 590__ $$a9.229$$b2020
000108297 591__ $$aNANOSCIENCE & NANOTECHNOLOGY$$b21 / 106 = 0.198$$c2020$$dQ1$$eT1
000108297 591__ $$aMATERIALS SCIENCE, MULTIDISCIPLINARY$$b44 / 333 = 0.132$$c2020$$dQ1$$eT1
000108297 592__ $$a2.535$$b2020
000108297 593__ $$aMaterials Science (miscellaneous)$$c2020$$dQ1
000108297 593__ $$aNanoscience and Nanotechnology$$c2020$$dQ1
000108297 593__ $$aMedicine (miscellaneous)$$c2020$$dQ1
000108297 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000108297 700__ $$0(orcid)0000-0002-0272-3152$$aGámez, Enrique
000108297 700__ $$0(orcid)0000-0003-1599-8216$$aLanda, Guillermo$$uUniversidad de Zaragoza
000108297 700__ $$0(orcid)0000-0003-3165-0156$$aArruebo, Manuel$$uUniversidad de Zaragoza
000108297 700__ $$0(orcid)0000-0002-2966-9088$$aIrusta, Silvia$$uUniversidad de Zaragoza
000108297 700__ $$0(orcid)0000-0003-2293-363X$$aMendoza, Gracia$$uUniversidad de Zaragoza
000108297 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000108297 7102_ $$15005$$2790$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Tecnologi. Medio Ambiente
000108297 773__ $$g12, 46 (2020), 51302-51313$$pACS appl. mater. interfaces$$tACS Applied Materials and Interfaces$$x1944-8244
000108297 8564_ $$s1780494$$uhttps://zaguan.unizar.es/record/108297/files/texto_completo.pdf$$yPostprint
000108297 8564_ $$s687759$$uhttps://zaguan.unizar.es/record/108297/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000108297 909CO $$ooai:zaguan.unizar.es:108297$$particulos$$pdriver
000108297 951__ $$a2021-11-15-08:54:15
000108297 980__ $$aARTICLE