000108331 001__ 108331
000108331 005__ 20211115135505.0
000108331 0247_ $$2doi$$a10.1105/tpc.20.00766
000108331 0248_ $$2sideral$$a122425
000108331 037__ $$aART-2020-122425
000108331 041__ $$aeng
000108331 100__ $$aLaureano-Marín, A.M.
000108331 245__ $$aAbscisic acid-triggered persulfidation of the cys protease ATG4 mediates regulation of autophagy by sulfide
000108331 260__ $$c2020
000108331 5060_ $$aAccess copy available to the general public$$fUnrestricted
000108331 5203_ $$aHydrogen sulfide is a signaling molecule that regulates essential processes in plants, such as autophagy. In Arabidopsis (Arabidopsis thaliana), hydrogen sulfide negatively regulates autophagy independently of reactive oxygen species via an unknown mechanism. Comparative and quantitative proteomic analysis was used to detect abscisic acid-triggered persulfidation that reveals a main role in the control of autophagy mediated by the autophagy-related (ATG) Cys protease AtATG4a. This protease undergoes specific persulfidation of Cys170 that is a part of the characteristic catalytic Cys-His-Asp triad of Cys proteases. Regulation of the ATG4 activity by persulfidation was tested in a heterologous assay using the Chlamydomonas reinhardtii CrATG8 protein as a substrate. Sulfide significantly and reversibly inactivates AtATG4a. The biological significance of the reversible inhibition of the ATG4 by sulfide is supported by the results obtained in Arabidopsis leaves under basal and autophagy-activating conditions. A significant increase in the overall ATG4 proteolytic activity in Arabidopsis was detected under nitrogen starvation and osmotic stress and can be inhibited by sulfide. Therefore, the data strongly suggest that the negative regulation of autophagy by sulfide is mediated by specific persulfidation of the ATG4 protease.
000108331 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FEDER/E35-17R$$9info:eu-repo/grantAgreement/EC/H2020/834120/EU/Regulation of Selective autophagy by sulfide through persulfidation of protein targets/SSHelectPhagy$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 834120-SSHelectPhagy
000108331 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000108331 590__ $$a11.277$$b2020
000108331 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b25 / 297 = 0.084$$c2020$$dQ1$$eT1
000108331 591__ $$aPLANT SCIENCES$$b6 / 235 = 0.026$$c2020$$dQ1$$eT1
000108331 591__ $$aCELL BIOLOGY$$b24 / 195 = 0.123$$c2020$$dQ1$$eT1
000108331 592__ $$a5.323$$b2020
000108331 593__ $$aPlant Science$$c2020$$dQ1
000108331 593__ $$aCell Biology$$c2020$$dQ1
000108331 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000108331 700__ $$aAroca, Á.
000108331 700__ $$aPérez-Pérez, M.E.
000108331 700__ $$0(orcid)0000-0003-3608-4720$$aYruela, I.$$uUniversidad de Zaragoza
000108331 700__ $$aJurado-Flores, A.
000108331 700__ $$aMoreno, I.
000108331 700__ $$aCrespo, J.L.
000108331 700__ $$aRomero, L.C.
000108331 700__ $$aGotor, C.
000108331 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000108331 773__ $$g32, 12 (2020), 3902-3920$$pPlant cell$$tPLANT CELL$$x1040-4651
000108331 8564_ $$s4050334$$uhttps://zaguan.unizar.es/record/108331/files/texto_completo.pdf$$yPostprint
000108331 8564_ $$s1934167$$uhttps://zaguan.unizar.es/record/108331/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000108331 909CO $$ooai:zaguan.unizar.es:108331$$particulos$$pdriver
000108331 951__ $$a2021-11-15-08:57:17
000108331 980__ $$aARTICLE