108331 20211115135505.0 doi 10.1105/tpc.20.00766 sideral 122425 ART-2020-122425 eng Laureano-Marín, A.M. Abscisic acid-triggered persulfidation of the cys protease ATG4 mediates regulation of autophagy by sulfide 2020 Hydrogen sulfide is a signaling molecule that regulates essential processes in plants, such as autophagy. In Arabidopsis (Arabidopsis thaliana), hydrogen sulfide negatively regulates autophagy independently of reactive oxygen species via an unknown mechanism. Comparative and quantitative proteomic analysis was used to detect abscisic acid-triggered persulfidation that reveals a main role in the control of autophagy mediated by the autophagy-related (ATG) Cys protease AtATG4a. This protease undergoes specific persulfidation of Cys170 that is a part of the characteristic catalytic Cys-His-Asp triad of Cys proteases. Regulation of the ATG4 activity by persulfidation was tested in a heterologous assay using the Chlamydomonas reinhardtii CrATG8 protein as a substrate. Sulfide significantly and reversibly inactivates AtATG4a. The biological significance of the reversible inhibition of the ATG4 by sulfide is supported by the results obtained in Arabidopsis leaves under basal and autophagy-activating conditions. A significant increase in the overall ATG4 proteolytic activity in Arabidopsis was detected under nitrogen starvation and osmotic stress and can be inhibited by sulfide. Therefore, the data strongly suggest that the negative regulation of autophagy by sulfide is mediated by specific persulfidation of the ATG4 protease. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/DGA-FEDER/E35-17R info:eu-repo/grantAgreement/EC/H2020/834120/EU/Regulation of Selective autophagy by sulfide through persulfidation of protein targets/SSHelectPhagy This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 834120-SSHelectPhagy info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 11.277 2020 BIOCHEMISTRY & MOLECULAR BIOLOGY 25 / 297 = 0.084 2020 Q1 T1 PLANT SCIENCES 6 / 235 = 0.026 2020 Q1 T1 CELL BIOLOGY 24 / 195 = 0.123 2020 Q1 T1 5.323 2020 Plant Science 2020 Q1 Cell Biology 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Aroca, Á. Pérez-Pérez, M.E. Yruela, I. Universidad de Zaragoza (orcid)0000-0003-3608-4720 Jurado-Flores, A. Moreno, I. Crespo, J.L. Romero, L.C. Gotor, C. 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 32, 12 (2020), 3902-3920 Plant cell PLANT CELL 1040-4651 4050334 http://zaguan.unizar.es/record/108331/files/texto_completo.pdf Postprint 1934167 http://zaguan.unizar.es/record/108331/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:108331 articulos driver 2021-11-15-08:57:17 ARTICLE