000108361 001__ 108361
000108361 005__ 20230519145416.0
000108361 0247_ $$2doi$$a10.1128/mBio.01129-21
000108361 0248_ $$2sideral$$a125042
000108361 037__ $$aART-2021-125042
000108361 041__ $$aeng
000108361 100__ $$aMortier, Julien
000108361 245__ $$aGene Erosion Can Lead to Gain-of-Function Alleles That Contribute to Bacterial Fitness
000108361 260__ $$c2021
000108361 5060_ $$aAccess copy available to the general public$$fUnrestricted
000108361 5203_ $$aDespite our extensive knowledge of the genetic regulation of heat shock proteins (HSPs), the evolutionary routes that allow bacteria to adaptively tune their HSP levels and corresponding proteostatic robustness have been explored less. In this report, directed evolution experiments using the Escherichia coli model system unexpectedly revealed that seemingly random single mutations in its tnaA gene can confer significant heat resistance. Closer examination, however, indicated that these mutations create folding-deficient and aggregation-prone TnaA variants that in turn can endogenously and preemptively trigger HSP expression to cause heat resistance. These findings, importantly, demonstrate that even erosive mutations with disruptive effects on protein structure and functionality can still yield true gain-of-function alleles with a selective advantage in adaptive evolution.
000108361 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000108361 590__ $$a7.786$$b2021
000108361 592__ $$a2.767$$b2021
000108361 594__ $$a11.2$$b2021
000108361 591__ $$aMICROBIOLOGY$$b22 / 138 = 0.159$$c2021$$dQ1$$eT1
000108361 593__ $$aVirology$$c2021$$dQ1
000108361 593__ $$aMicrobiology$$c2021$$dQ1
000108361 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000108361 700__ $$0(orcid)0000-0002-5895-2157$$aGayán, Elisa$$uUniversidad de Zaragoza
000108361 700__ $$aVan Eyken, Ronald
000108361 700__ $$aTorres Montaguth
000108361 700__ $$aOscar Enrique
000108361 700__ $$aKhodaparast, Ladan
000108361 700__ $$aKhodaparast, Laleh
000108361 700__ $$aHouben, Bert
000108361 700__ $$aCarpentier, Sebastien
000108361 700__ $$aRousseau, Frederic
000108361 700__ $$aSchymkowitz, Joost
000108361 700__ $$aAertsen, Abram
000108361 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000108361 773__ $$g12, 4 (2021), 01129-21 [21 pp.]$$pMBIO$$tMBIO$$x2161-2129
000108361 8564_ $$s3242912$$uhttps://zaguan.unizar.es/record/108361/files/texto_completo.pdf$$yVersión publicada
000108361 8564_ $$s2467977$$uhttps://zaguan.unizar.es/record/108361/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000108361 909CO $$ooai:zaguan.unizar.es:108361$$particulos$$pdriver
000108361 951__ $$a2023-05-18-14:00:38
000108361 980__ $$aARTICLE