108361 20230519145416.0 doi 10.1128/mBio.01129-21 sideral 125042 ART-2021-125042 eng Mortier, Julien Gene Erosion Can Lead to Gain-of-Function Alleles That Contribute to Bacterial Fitness 2021 Access copy available to the general public Unrestricted Despite our extensive knowledge of the genetic regulation of heat shock proteins (HSPs), the evolutionary routes that allow bacteria to adaptively tune their HSP levels and corresponding proteostatic robustness have been explored less. In this report, directed evolution experiments using the Escherichia coli model system unexpectedly revealed that seemingly random single mutations in its tnaA gene can confer significant heat resistance. Closer examination, however, indicated that these mutations create folding-deficient and aggregation-prone TnaA variants that in turn can endogenously and preemptively trigger HSP expression to cause heat resistance. These findings, importantly, demonstrate that even erosive mutations with disruptive effects on protein structure and functionality can still yield true gain-of-function alleles with a selective advantage in adaptive evolution. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 7.786 2021 MICROBIOLOGY 22 / 138 = 0.159 2021 Q1 T1 2.767 2021 Virology 2021 Q1 Microbiology 2021 Q1 11.2 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Gayán, Elisa Universidad de Zaragoza (orcid)0000-0002-5895-2157 Van Eyken, Ronald Torres Montaguth Oscar Enrique Khodaparast, Ladan Khodaparast, Laleh Houben, Bert Carpentier, Sebastien Rousseau, Frederic Schymkowitz, Joost Aertsen, Abram 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 12, 4 (2021), 01129-21 [21 pp.] MBIO MBIO 2161-2129 3242912 http://zaguan.unizar.es/record/108361/files/texto_completo.pdf Versión publicada 2467977 http://zaguan.unizar.es/record/108361/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:108361 articulos driver 2023-05-18-14:00:38 ARTICLE