000108377 001__ 108377
000108377 005__ 20220208112851.0
000108377 0247_ $$2doi$$a10.1002/2211-5463.13206
000108377 0248_ $$2sideral$$a124996
000108377 037__ $$aART-2021-124996
000108377 041__ $$aeng
000108377 100__ $$0(orcid)0000-0002-5797-3909$$aLatorre, Eva$$uUniversidad de Zaragoza
000108377 245__ $$aMicrobiota-derived short-chain fatty acids as modulators of intestinal serotonin transporter
000108377 260__ $$c2021
000108377 5060_ $$aAccess copy available to the general public$$fUnrestricted
000108377 5203_ $$aSerotonin is a key neuromodulator of intestinal physiology. Serotonin transporter (SERT) is responsible for serotonin uptake, modulating its availability and consequently, serotonergic signalling. Recently, microbiota has been described to affect intestinal homeostasis through microbiota recognition receptors (TLRs). In fact,TLRs activation seems to regulate intestinal serotonergic system. However, whether intestinal microbiota can modulate SERT by short-chain fatty acids (SCFA) is unknown. Microbiota-derived short-chain fatty acids such as acetate, propionate and butyrate, are important metabolites from non-digestible dietary fibers bacterial fermentation. These metabolites have been shown to maintain intestinal homeostasis through protecting epithelial barrier integrity, promoting IgA production and regulating T-cell differentiation. In this study human enterocyte-like Caco-2/TC7 cells were used as intestinal epithelial cells model, which expresses serotonin transporter. Caco-2/TC7 cells were treated for 24 h with different concentrations of acetate, propionate and butyrate and then, and 5-HT uptake was measured. SERT molecular expression was analysed by measuring both, mRNA levels by real-time PCR and protein expression by western blotting. Our results show that the treatment with SCFA modulates SERT function and expression, in a different way for each fatty acid. Consequently, a different production of SCFA by microbiota could differently modulate SERT and affect to serotonergic signalling and intestinal physiology. Our study contributes to growing evidence about the key role of microbiota on host physiology regulation, and it opens a cutting-edge opportunity of microbiota modulation to balance serotonergic signalling alterations.
000108377 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000108377 655_4 $$ainfo:eu-repo/semantics/conferenceObject$$vinfo:eu-repo/semantics/publishedVersion
000108377 700__ $$aForcén, Ana
000108377 700__ $$aBuey, Berta
000108377 700__ $$0(orcid)0000-0003-4758-3998$$aGrasa, Laura$$uUniversidad de Zaragoza
000108377 700__ $$0(orcid)0000-0002-8467-0356$$aMesonero, José Emilio
000108377 7102_ $$11005$$2410$$aUniversidad de Zaragoza$$bDpto. Farmacología y Fisiolog.$$cÁrea Fisiología
000108377 7102_ $$11002$$2050$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Biología Celular
000108377 773__ $$g11 (2021), 46-47$$pFEBS openbio$$tFEBS openbio$$x2211-5463
000108377 8564_ $$s75569$$uhttps://zaguan.unizar.es/record/108377/files/texto_completo.pdf$$yVersión publicada
000108377 8564_ $$s3211658$$uhttps://zaguan.unizar.es/record/108377/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000108377 909CO $$ooai:zaguan.unizar.es:108377$$particulos$$pdriver
000108377 951__ $$a2022-02-08-11:25:31
000108377 980__ $$aARTICLE