108430 20230602121506.0 sideral 125001 ART-2021-125001 eng Bellés, A. Universidad de Zaragoza (orcid)0000-0002-6546-6149 Native and saturated bovine lactoferrin modulate the expression of toll-like receptors (TLRS) in mice treated with antibiotics. P0203. 29th United European Gastroenterology Week Virtual 2021 2021 Access copy available to the general public Unrestricted Introduction: The gut microbiota plays a fundamental role in protection against pathogen infections but also in the regulation of the immune sys-tem. The antibiotic administration can result in gut microbiota alterations and changes in the eectiveness of innate immune responses. As micro-biota composition changes, the altered community will present dierent microbial-associated molecular patterns to Toll-like receptors (TLRs), which can cascade down through various immune responses, including changes in the expression of TLR receptors [1]. In this context, finding ingredients that can modulate the immune system is becoming a major focus of interest to produce functional foods. Bovine lactoferrin is a milk protein with many biological properties, including an-tibacterial and immunomodulatory activity [2].Aims & Methods: The objective was to determine the eects of native (nLf) and saturated (sLf) bovine lactoferrin on TLR receptor expression of colon from mice treated or untreated with the antibiotic clindamycin, to evalu-ate its potential to be added in functional foods.Male C57BL/6 mice of 8 weeks old were randomly divided into six groups (n=5 per group): vehicle, clindamycin (Clin), native bovine lactoferrin (nLf), nLf + clindamycin (nLf_Clin), iron-saturated bovine lactoferrin (sLf) and sLf + clindamycin (sLf_Clin). Vehicle received saline orally for 10 days. Clin was gavaged for 10 days with saline and on day 4 received a single IP injection of 200 µg of clindamycin. nLf and sLf were gavaged for 10 days with 35 mg of nLf or sLf. The groups nLf_Clin and sLf_Clin were gavaged with nLf or sLf and on day 4 received an injection of clindamycin.The gene expression (mRNA) of TLR1-9 receptors was determined in the colon from mice by RT-PCR and relative expression levels of genes were calculated using the 2 -∆∆CT method.Results: The expression of TLR2, TLR4, TLR5 and TLR6 was not modified in any of the treated groups. The expression of TLR1 was increased in sLf, nLf_Clin and sLf_Clin treated groups, indicating that the eect of sLf on TLR1 expression was maintained despite the treatment with Clin. The expression of TLR8 and TLR9 decreased in Clin, nLf and nLf_Clin groups. However, the levels of these receptors in sLf_Clin were similar to Vehicle, demonstrating the immunomodulatory capacity of sLf to restore these receptor levels, in a situation of intestinal dysbiosis. Finally, respect to TLR3 and TLR7, only the group of mice treated with sLf_Clin showed an increase in the expression of this receptor. Conclusion: Saturated bovine lactoferrin can restore the expression levels of TLR8 and TLR9 in conditions of intestinal dysbiosis induced by antibiot-ics. These results confirm the immunomodulatory properties of lactoferrin, which have great interest for the design of functional foods. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 6.866 2021 GASTROENTEROLOGY & HEPATOLOGY 23 / 92 = 0.25 2021 Q1 T1 1.445 2021 Oncology 2021 Q1 Gastroenterology 2021 Q1 7.9 2021 info:eu-repo/semantics/conferenceObject info:eu-repo/semantics/publishedVersion Aguirre-Ramírez, D. (orcid)0000-0002-1829-6319 Abad, I. Universidad de Zaragoza Sánchez, L. Universidad de Zaragoza (orcid)0000-0001-5964-823X Grasa, L. Universidad de Zaragoza (orcid)0000-0002-5306-9365 1012 410 Universidad de Zaragoza Dpto. Farmac.Fisiol.y Med.L.F. Área Fisiología 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 9, 58 (2021), 376 United European Gastroenterol. j. United European Gastroenterology Journal 2050-6406 202809 http://zaguan.unizar.es/record/108430/files/texto_completo.pdf Versión publicada 1717835 http://zaguan.unizar.es/record/108430/files/texto_completo.jpg?subformat=icon icon Versión publicada https://onlinelibrary.wiley.com/doi/epdf/10.1002/ueg2.12144 Texto completo de la revista oai:zaguan.unizar.es:108430 articulos driver 2023-06-02-11:02:43 ARTICLE