108505 20211122153226.0 doi 10.1021/acs.inorgchem.0c02922 sideral 122422 ART-2020-122422 eng Mármol, I. Gold(I) and Silver(I) Complexes with 2-Anilinopyridine-Based Heterocycles as Multitarget Drugs against Colon Cancer 2020 A series of gold(I) and silver(I) derivatives with N- or S-donor ligands derived from 2-anilinopyridine has been synthesized and characterized. The mononuclear structure of [Au(L1)(PPh3)](TfO) (1a) and [Au(L2)(PPh3)](TfO) (1b) was confirmed by X-ray diffraction studies, as well as the dinuclear structure in the case of [Ag(TfO)(L1)]2 (4a). Most of the complexes are cytotoxic against a model of colorectal adenocarcinoma (Caco-2 cell line) and breast adenocarcinoma cancer cell lines (MCF-7). [Au(L1)(PPh3)](TfO) (1a) was able to induce caspases 8 and 3 activation, loss of mitochondrial membrane potential, and reactive oxygen species (ROS)-dependent cell death on Caco-2 cells upon 24 h incubation. In addition, the gold complex 1a produced a significant inhibition of the redox enzyme thioredoxin reductase as well as 20S proteasome. However, the silver(I) analogue, [Ag(TfO)(L1)(PPh3)] (2a), induced cell death independent of inhibition of thioredoxin reductase and 20S proteasome, triggered ROS-independent apoptosis mediated by caspase 8 and 3 activation, and loss of mitochondrial membrane potential, which points to a different mechanism of action for both derivatives, dependent on the metal center. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/DGA/B16-R17 info:eu-repo/grantAgreement/ES/DGA-FSE/E07-20R info:eu-repo/grantAgreement/ES/ISCIII-CIBERObn/CB06-03-1012 info:eu-repo/grantAgreement/ES/MCIU/RED2018-102471-T info:eu-repo/grantAgreement/ES/MINECO/CTQ2016-75816-C2-1-P info:eu-repo/grantAgreement/ES/MINECO/PID2019-104379RB-C21 info:eu-repo/grantAgreement/ES/MINECO/SAF2016-75441-R info:eu-repo/grantAgreement/EUR/SUDOE/INTERREG/Redvalue-SOE1-PI-E0123 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 5.165 2020 CHEMISTRY, INORGANIC & NUCLEAR 5 / 45 = 0.111 2020 Q1 T1 1.348 2020 Chemistry (miscellaneous) 2020 Q1 Physical and Theoretical Chemistry 2020 Q1 Inorganic Chemistry 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Montanel-Perez, S. Royo, J.C. Gimeno, M.C. Universidad de Zaragoza (orcid)0000-0003-0553-0695 Villacampa, M.D. (orcid)0000-0002-3978-1145 Rodríguez-Yoldi, M.J. Universidad de Zaragoza (orcid)0000-0002-3595-7668 Cerrada, E. Universidad de Zaragoza (orcid)0000-0003-2457-3674 2010 760 Universidad de Zaragoza Dpto. Química Inorgánica Área Química Inorgánica 1005 410 Universidad de Zaragoza Dpto. Farmacología y Fisiolog. Área Fisiología 59, 23 (2020), 17732-17745 Inorg. chem. Inorganic Chemistry 0020-1669 781817 http://zaguan.unizar.es/record/108505/files/texto_completo.pdf Postprint 1693308 http://zaguan.unizar.es/record/108505/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:108505 articulos driver 2021-11-22-14:00:35 ARTICLE