000108567 001__ 108567
000108567 005__ 20230519145503.0
000108567 0247_ $$2doi$$a10.1063/5.0061997
000108567 0248_ $$2sideral$$a125112
000108567 037__ $$aART-2021-125112
000108567 041__ $$aeng
000108567 100__ $$0(orcid)0000-0003-1210-8732$$aPérez Rodríguez, S.$$uUniversidad de Zaragoza
000108567 245__ $$aMicrofluidic model of monocyte extravasation reveals the role of hemodynamics and subendothelial matrix mechanics in regulating endothelial integrity
000108567 260__ $$c2021
000108567 5060_ $$aAccess copy available to the general public$$fUnrestricted
000108567 5203_ $$aExtravasation of circulating cells is an essential process that governs tissue inflammation and the body's response to pathogenic infection. To initiate anti-inflammatory and phagocytic functions within tissues, immune cells must cross the vascular endothelial barrier from the vessel lumen to the subluminal extracellular matrix. In this work, we present a microfluidic approach that enables the recreation of a three-dimensional, perfused endothelial vessel formed by human endothelial cells embedded within a collagen-rich matrix. Monocytes are introduced into the vessel perfusate, and we investigate the role of luminal flow and collagen concentration on extravasation. In vessels conditioned with the flow, increased monocyte adhesion to the vascular wall was observed, though fewer monocytes extravasated to the collagen hydrogel. Our results suggest that the lower rates of extravasation are due to the increased vessel integrity and reduced permeability of the endothelial monolayer. We further demonstrate that vascular permeability is a function of collagen hydrogel mass concentration, with increased collagen concentrations leading to elevated vascular permeability and increased extravasation. Collectively, our results demonstrate that extravasation of monocytes is highly regulated by the structural integrity of the endothelial monolayer. The microfluidic approach developed here allows for the dissection of the relative contributions of these cues to further understand the key governing processes that regulate circulating cell extravasation and inflammation.
000108567 536__ $$9info:eu-repo/grantAgreement/ES/MEC/FPU16-04398$$9info:eu-repo/grantAgreement/ES/MICINN/RTI2018-094494-B-C21
000108567 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000108567 590__ $$a3.258$$b2021
000108567 592__ $$a0.584$$b2021
000108567 594__ $$a5.5$$b2021
000108567 591__ $$aPHYSICS, FLUIDS & PLASMAS$$b12 / 34 = 0.353$$c2021$$dQ2$$eT2
000108567 593__ $$aBiomedical Engineering$$c2021$$dQ2
000108567 591__ $$aBIOPHYSICS$$b40 / 72 = 0.556$$c2021$$dQ3$$eT2
000108567 593__ $$aCondensed Matter Physics$$c2021$$dQ2
000108567 591__ $$aNANOSCIENCE & NANOTECHNOLOGY$$b76 / 109 = 0.697$$c2021$$dQ3$$eT3
000108567 593__ $$aGenetics$$c2021$$dQ2
000108567 591__ $$aBIOCHEMICAL RESEARCH METHODS$$b40 / 79 = 0.506$$c2021$$dQ3$$eT2
000108567 593__ $$aPhysical and Theoretical Chemistry$$c2021$$dQ2
000108567 593__ $$aMolecular Biology$$c2021$$dQ2
000108567 593__ $$aNanoscience and Nanotechnology$$c2021$$dQ2
000108567 593__ $$aMaterials Science (miscellaneous)$$c2021$$dQ2
000108567 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000108567 700__ $$aHuang, S.A.
000108567 700__ $$0(orcid)0000-0002-3784-1140$$aBorau, C.$$uUniversidad de Zaragoza
000108567 700__ $$0(orcid)0000-0002-9864-7683$$aGarcía Aznar, J.M.$$uUniversidad de Zaragoza
000108567 700__ $$aPolacheck, W.J.
000108567 7102_ $$11011$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Microbiología
000108567 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est.
000108567 773__ $$g15, 5 (2021), 054102 [12 pp.]$$pBiomicrofluidics$$tBiomicrofluidics$$x1932-1058
000108567 8564_ $$s7018556$$uhttps://zaguan.unizar.es/record/108567/files/texto_completo.pdf$$yVersión publicada
000108567 8564_ $$s1727959$$uhttps://zaguan.unizar.es/record/108567/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000108567 909CO $$ooai:zaguan.unizar.es:108567$$particulos$$pdriver
000108567 951__ $$a2023-05-18-15:02:37
000108567 980__ $$aARTICLE