108567 20230519145503.0 doi 10.1063/5.0061997 sideral 125112 ART-2021-125112 eng Pérez Rodríguez, S. Universidad de Zaragoza (orcid)0000-0003-1210-8732 Microfluidic model of monocyte extravasation reveals the role of hemodynamics and subendothelial matrix mechanics in regulating endothelial integrity 2021 Access copy available to the general public Unrestricted Extravasation of circulating cells is an essential process that governs tissue inflammation and the body's response to pathogenic infection. To initiate anti-inflammatory and phagocytic functions within tissues, immune cells must cross the vascular endothelial barrier from the vessel lumen to the subluminal extracellular matrix. In this work, we present a microfluidic approach that enables the recreation of a three-dimensional, perfused endothelial vessel formed by human endothelial cells embedded within a collagen-rich matrix. Monocytes are introduced into the vessel perfusate, and we investigate the role of luminal flow and collagen concentration on extravasation. In vessels conditioned with the flow, increased monocyte adhesion to the vascular wall was observed, though fewer monocytes extravasated to the collagen hydrogel. Our results suggest that the lower rates of extravasation are due to the increased vessel integrity and reduced permeability of the endothelial monolayer. We further demonstrate that vascular permeability is a function of collagen hydrogel mass concentration, with increased collagen concentrations leading to elevated vascular permeability and increased extravasation. Collectively, our results demonstrate that extravasation of monocytes is highly regulated by the structural integrity of the endothelial monolayer. The microfluidic approach developed here allows for the dissection of the relative contributions of these cues to further understand the key governing processes that regulate circulating cell extravasation and inflammation. info:eu-repo/grantAgreement/ES/MEC/FPU16-04398 info:eu-repo/grantAgreement/ES/MICINN/RTI2018-094494-B-C21 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.258 2021 PHYSICS, FLUIDS & PLASMAS 12 / 34 = 0.353 2021 Q2 T2 BIOPHYSICS 40 / 72 = 0.556 2021 Q3 T2 NANOSCIENCE & NANOTECHNOLOGY 76 / 109 = 0.697 2021 Q3 T3 BIOCHEMICAL RESEARCH METHODS 40 / 79 = 0.506 2021 Q3 T2 0.584 2021 Biomedical Engineering 2021 Q2 Condensed Matter Physics 2021 Q2 Genetics 2021 Q2 Physical and Theoretical Chemistry 2021 Q2 Molecular Biology 2021 Q2 Nanoscience and Nanotechnology 2021 Q2 Materials Science (miscellaneous) 2021 Q2 5.5 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Huang, S.A. Borau, C. Universidad de Zaragoza (orcid)0000-0002-3784-1140 García Aznar, J.M. Universidad de Zaragoza (orcid)0000-0002-9864-7683 Polacheck, W.J. 1011 630 Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Microbiología 5004 605 Universidad de Zaragoza Dpto. Ingeniería Mecánica Área Mec.Med.Cont. y Teor.Est. 15, 5 (2021), 054102 [12 pp.] Biomicrofluidics Biomicrofluidics 1932-1058 7018556 http://zaguan.unizar.es/record/108567/files/texto_completo.pdf Versión publicada 1727959 http://zaguan.unizar.es/record/108567/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:108567 articulos driver 2023-05-18-15:02:37 ARTICLE