000108652 001__ 108652 000108652 005__ 20211216104750.0 000108652 037__ $$aTAZ-TFM-2021-524 000108652 041__ $$aeng 000108652 1001_ $$aMatute Lamana, Carlos 000108652 24200 $$aCo-cultured neurons and microglia treated with neuromelanin as a model to study the events leading to protein aggregation in Parkinson's disease. 000108652 24500 $$aCo-cultivo de neuronas y microglía tratadas con neuromelanina como modelo de estudio de los procesos involucrados en la agregación de proteínas en la enfermedad del Parkinson 000108652 260__ $$aZaragoza$$bUniversidad de Zaragoza$$c2021 000108652 506__ $$aby-nc-sa$$bCreative Commons$$c3.0$$uhttp://creativecommons.org/licenses/by-nc-sa/3.0/ 000108652 520__ $$aParkinson's disease is a common neurodegenerative disease that affects more than 10 million people worldwide which causes motor abnormalities such as tremor, muscle stiffness, paucity of voluntary movements and postural instability. It is mainly characterized by neural degeneration in the substantia nigra of the mid brain. Misfolding and aggregation of alpha-synuclein (aSyn) is one of the major molecular hallmarks of the disease but it is not clear which are the initial molecular and cellular mechanisms leading to protein aggregation, although cellular stress caused by oxidative damage with the involvement of neuromelanin and microglia-mediated inflammatory responses appear to be involved. No experimental model has been able so far to accurately replicate the situation within the human brain. In this work, we have used co-cultures of in vitro-differentiated dopaminergic neurons (SH-SY5Y) together with an immortalized human microglia cell line (HMC3), treated with synthetic neuromelanin, to approach the initial processes leading to aSyn aggregation using 2D models. <br /><br /> 000108652 521__ $$aMáster Universitario en Biotecnología Cuantitativa 000108652 540__ $$aDerechos regulados por licencia Creative Commons 000108652 700__ $$aCarrodeguas Villar, José Alberto$$edir. 000108652 700__ $$aDe la Fuente Herreruela, Diego$$edir. 000108652 7102_ $$aUniversidad de Zaragoza$$bBioquímica y Biología Molecular y Celular$$cBioquímica y Biología Molecular 000108652 8560_ $$f736786@unizar.es 000108652 8564_ $$s4848259$$uhttps://zaguan.unizar.es/record/108652/files/TAZ-TFM-2021-524.pdf$$yMemoria (eng) 000108652 909CO $$ooai:zaguan.unizar.es:108652$$pdriver$$ptrabajos-fin-master 000108652 950__ $$a 000108652 951__ $$adeposita:2021-12-16 000108652 980__ $$aTAZ$$bTFM$$cCIEN 000108652 999__ $$a20210628124311.CREATION_DATE